Parks O W, Doerr R C
J Assoc Off Anal Chem. 1986 Jan-Feb;69(1):70-1.
A procedure is described for the quantitation of Zoalene (3,5-dinitro-o-toluamide) and its 2 major monoamino metabolites in chicken tissues. The method includes blender extraction of tissue with chloroformethyl acetate (1 + 1), adsorption of the drug and metabolites on neutral alumina, and subsequent elution of the residues with pH 3.5 formate buffer-methanol (6.5 + 3.5). Recovered residues were separated on a 5 micron C18 column with the alumina eluting solvent as the LC mobile phase. The parent drug and metabolites were detected and quantitated with an electrochemical detector in the reductive mode with a minimum level of reliable measurement of 0.1 ppm. Overall mean recoveries greater than 85% were obtained with Zoalene and its 2 monoamino metabolites in breast, thigh, and liver tissues fortified with 0.25-2.00 ppm. The results on tissues from chickens fed a diet containing 0.0125% Zoalene are presented.
本文描述了一种定量测定鸡肉组织中唑啉（3,5 - 二硝基 - 邻甲苯酰胺）及其两种主要单氨基代谢物的方法。该方法包括用氯仿 - 乙酸乙酯（1 + 1）对组织进行匀浆提取，将药物和代谢物吸附在中性氧化铝上，随后用pH 3.5的甲酸盐缓冲液 - 甲醇（6.5 + 3.5）洗脱残留物。将回收的残留物在5微米的C18柱上进行分离，以氧化铝洗脱溶剂作为液相色谱流动相。采用电化学检测器在还原模式下对母体药物和代谢物进行检测和定量，可靠测量的最低水平为0.1 ppm。在添加了0.25 - 2.00 ppm的胸肌、大腿和肝脏组织中，唑啉及其两种单氨基代谢物的总体平均回收率大于85%。文中还给出了喂食含0.0125%唑啉日粮的鸡的组织检测结果。
