Parks O W
U.S. Department of Agriculture, Philadelphia, PA 19119.
J Assoc Off Anal Chem. 1989 Jul-Aug;72(4):567-9.
A screening procedure is described for the detection of furazolidone, nitrofurazone, aklomide, zoalene, nitromide, and sulfanitran residues in a single extract of chicken liver, breast, or thigh muscle at the low ppb level. The method includes extraction of tissue with chloroformethyl acetate-dimethyl sulfoxide (50 + 50 + 0.8), adsorption on neutral alumina, and subsequent elution of the residues with pH 6.0 phosphate buffer-methanol (1 + 1). Eluants are separated on a 25 cm, 5 microns C18 column with pH 6.0 phosphate buffer-methanol (57.5 + 42.5) as mobile phase. The drugs are detected with an electrochemical detector in the reductive mode at -0.8 V. Mean recoveries from all tissues ranged from 76.5% for nitrofurazone to 97.1% for zoalene.
本文描述了一种筛查程序，用于检测鸡肝、鸡胸肉或鸡腿肉的单一提取物中低至十亿分比（ppb）水平的呋喃唑酮、呋喃西林、阿克洛胺、佐胺、硝米特和磺胺硝苯残留。该方法包括用氯仿 - 乙酸乙酯 - 二甲基亚砜（50 + 50 + 0.8）提取组织，吸附于中性氧化铝上，随后用pH 6.0的磷酸盐缓冲液 - 甲醇（1 + 1）洗脱残留。洗脱液在一根25 cm、5微米的C18柱上进行分离，以pH 6.0的磷酸盐缓冲液 - 甲醇（57.5 + 42.5）作为流动相。使用电化学检测器在 -0.8 V的还原模式下检测这些药物。所有组织的平均回收率范围为呋喃西林的76.5%至佐胺的97.1%。
