Lv Yao, Xie Xianjing, Shi Hong, Guo Yuna
The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China.
Heliyon. 2024 Oct 1;10(20):e38788. doi: 10.1016/j.heliyon.2024.e38788. eCollection 2024 Oct 30.
Untargeted metabolomics can be used for the comprehensive analysis of metabolite profiles in biological samples without preset targets, making them particularly suitable for exploring metabolic characteristics and potential mechanisms in complex diseases. Therefore, in this study, we employed gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) techniques to analyze the serum metabolic characteristics of patients with pregnancy-associated venous thromboembolism (PA-VTE). In this study, 11 pregnant women with VTE and 11 healthy pregnant women were included in the experimental and control groups, respectively. Using GC-MS, we identified 325 metabolites, with the highest proportion being organic oxygen compounds. Using LC-MS, we identified 3104 metabolites, with the highest proportion being acylcarnitine. The results revealed significant differences in the levels of lipids, organic compounds, and other metabolites between patients compared to healthy pregnant women. Pathways such as pyrimidine metabolism, linoleic acid metabolism, and mineral absorption differed between patients with PA-VTE and controls. Furthermore, we identified biomarkers associated with metabolic processes, such as fatty acids and amino acids (2-hydroxyhexanedioic acid, hexadecenal, palmitoylethanolamide, glycerol-1-phosphate, and N-acetyl-beta-D-glucosamine). These findings revealed the metabolic characteristics of PA-VTE and provided important clues for further research on its pathophysiological mechanisms. Our findings may contribute to the development of new diagnostic markers and support early diagnosis and treatment of PA-VTE.
非靶向代谢组学可用于对生物样品中的代谢物谱进行全面分析，无需预设目标，这使得它们特别适用于探索复杂疾病中的代谢特征和潜在机制。因此，在本研究中，我们采用气相色谱 - 质谱联用（GC-MS）和液相色谱 - 质谱联用（LC-MS）技术来分析妊娠相关静脉血栓栓塞症（PA-VTE）患者的血清代谢特征。在本研究中，分别将11例患有VTE的孕妇和11例健康孕妇纳入实验组和对照组。使用GC-MS，我们鉴定出325种代谢物，其中比例最高的是有机氧化合物。使用LC-MS，我们鉴定出3104种代谢物，其中比例最高的是酰基肉碱。结果显示，与健康孕妇相比，患者的脂质、有机化合物和其他代谢物水平存在显著差异。PA-VTE患者与对照组之间嘧啶代谢、亚油酸代谢和矿物质吸收等途径有所不同。此外，我们鉴定出了与代谢过程相关的生物标志物，如脂肪酸和氨基酸（2-羟基己二酸、十六碳烯醛、棕榈酰乙醇胺、甘油-1-磷酸和N-乙酰-β-D-葡萄糖胺）。这些发现揭示了PA-VTE的代谢特征，并为进一步研究其病理生理机制提供了重要线索。我们的发现可能有助于开发新的诊断标志物，并支持PA-VTE的早期诊断和治疗。
