Saleem Usama, Asrar Muhammad, Jabeen Farhat, Makhdoom Hussain Syed, Hussain Dilbar
Department of Zoology, Faculty of Life Sciences, Government College University, Faisalabad, Punjab, Pakistan.
Entomological Research Institute, Ayub Agricultural Research Institute, Faisalabad, Punjab, Pakistan.
Heliyon. 2024 Oct 18;10(20):e39593. doi: 10.1016/j.heliyon.2024.e39593. eCollection 2024 Oct 30.
The fall armyworm, (J.E. Smith), is a devastating pest that attacks a wide range of crops, including sugarcane, rice, and maize. The purpose of this study is to evaluate the toxicity potential of native plant extracts (, ) against Four different concentrations (50, 100, 200, and 400 ppm) of the ethanolic plant extracts was evaluated against third-instar larvae to determine their median lethal concentration (LC). After 72 h of exposure, the LC values of , , and positive control (Spinetoram) were 186.104, 518.438, 320.027, 334.259, 252.651, 720.980 and 189.369 ppm respectively. The maximum percent mortality was caused by the highest concentration (400 ppm) of (64 ± 0.18), (48 ± 0.22) (56 ± 0.18), (56 ± 0.18), (60 ± 0.00), (40 ± 0.28), after 72 h of treatment while Spinetoram induced 100 ± 0.00 percent mortality of and only 4 ± 0.18 percent mortality was recorded in a control group. Results showed that all plant extracts were found to be effective against The compounds from the two most effective ethanolic plant extracts were identified by using Gas chromatography-mass spectrometry analysis (GC-MS). The key compounds identified in neem leaf extract and kortuma fruit extract are predominantly biologically active molecules. Many of them were volatile compounds that belonged to different chemical categories, such as fatty acids, hydrocarbons, esters, terpenoids, phenolic compounds, and amines. Terpenes exhibited a wide range of different biological activities, such as serving as insecticides and antifeedant. The presence of various functional groups in the plant extract was determined by conducting a Fourier Transform Infrared Spectroscopy (FTIR). Farmers should employ these kinds of environmental friendly insecticides to lessen the impact of fall armyworm because these products are cheaper to use and better for the economy and the environment.
草地贪夜蛾(J.E. 史密斯)是一种极具破坏力的害虫,会侵袭包括甘蔗、水稻和玉米在内的多种作物。本研究的目的是评估本地植物提取物([植物名称1]、[植物名称2])对草地贪夜蛾的潜在毒性。针对草地贪夜蛾三龄幼虫,评估了四种不同浓度(50、100、200和400 ppm)的植物乙醇提取物,以确定它们的半数致死浓度(LC)。暴露72小时后,[植物名称1]、[植物名称2]以及阳性对照(乙基多杀菌素)的LC值分别为186.104、518.438、320.027、334.259、252.651、720.980和189.369 ppm。处理72小时后,最高浓度(400 ppm)的[植物名称1]、[植物名称2]、[植物名称3]、[植物名称4]、[植物名称5]、[植物名称6]分别导致的最高死亡率为(64 ± 0.18)、(48 ± 0.22)、(56 ± 0.18)、(56 ± 0.18)、(60 ± 0.00)、(40 ± 0.28),而乙基多杀菌素导致草地贪夜蛾的死亡率为100 ± 0.00,对照组仅记录到4 ± 0.18的死亡率。结果表明,所有植物提取物对草地贪夜蛾均有效。通过气相色谱 - 质谱分析(GC - MS)鉴定了两种最有效的植物乙醇提取物中的化合物。印楝叶提取物和科尔图马果实提取物中鉴定出的关键化合物主要是生物活性分子。其中许多是属于不同化学类别的挥发性化合物,如脂肪酸、烃类、酯类、萜类、酚类化合物和胺类。萜类表现出广泛的不同生物活性,如作为杀虫剂和拒食剂。通过傅里叶变换红外光谱(FTIR)确定了植物提取物中各种官能团的存在。农民应使用这类环保型杀虫剂来减轻草地贪夜蛾的影响,因为这些产品使用成本更低,对经济和环境更有益。