Idries Ahmed H, Naser Eva H, Dafalla Maha B, Elmubarak Sara A A, Abdelrahim Yusria E, Abdalrhman Entsar A, Alwali Sabri Mustafa, Ahmed Bashir M, Yousef Bashir A, Ebrahim Reem M A, Abdellatif Ashraf O, Awadallah Amna K E, Osman Makarim Elfadil M, Konozy Emadeldin H E
Biotechnology Department, Africa City of Technology, Khartoum, Sudan.
Medicinal, Aromatic Plants and Traditional Medicine Research Institute (MAPTRI), National Center for Research, Khartoum, Sudan.
Heliyon. 2024 Oct 18;10(20):e39351. doi: 10.1016/j.heliyon.2024.e39351. eCollection 2024 Oct 30.
Fresen, an African medicinal plant, is known for its analgesic, antiulcer, and antimicrobial properties, with its leaves, bark, and fruits deeply ingrained in indigenous healing practices. Two lectins, TerBLL (from leaves) and TerBSL (from seeds) of Fresen, were purified using salting-out and affinity chromatography on a fetuin-agarose column. The purified lectins were then assessed for protein yield, hemagglutination activity, and physicochemical properties. Both TerBLL and TerBSL have subunits with molecular weights of 57.3 and 65.7 kDa, respectively. TerBLL remains stable at 60-80 °C and is activated by Mn, while TerBSL is activated by Zn. These lectins maintain consistent activity under acidic conditions, with TerBLL demonstrating heightened activity at extreme alkaline pH. TerBLL retained 50 % of its activity in 2-8M urea, in contrast to the 13 % of TerBSL. Investigation of the properties of TerBLL revealed that it had antinociceptive effects, reducing abdominal pain and prolonging latency time in the hotplate assay, potentially through μ-opioid receptor blockade akin to that of morphine. TerBLL exhibits antiulcer activity at doses of 0.25 and 1 mg/kg, reducing ulcer formation by up to 33 %, comparable to that of pantoprazole (80 mg/kg). The physiochemical attributes of TerBLL, in addition to its pain-relieving and gastroprotective effects, underscore its therapeutic promise, which is consistent with its traditional use.
弗雷森是一种非洲药用植物，以其镇痛、抗溃疡和抗菌特性而闻名，其叶子、树皮和果实深深融入当地的治疗方法中。从弗雷森中提取的两种凝集素，即来自叶子的TerBLL和来自种子的TerBSL，通过盐析和在胎球蛋白-琼脂糖柱上的亲和色谱法进行纯化。然后对纯化后的凝集素进行蛋白质产量、血凝活性和理化性质评估。TerBLL和TerBSL的亚基分子量分别为57.3 kDa和65.7 kDa。TerBLL在60-80°C下保持稳定，并被锰激活，而TerBSL被锌激活。这些凝集素在酸性条件下保持一致的活性，TerBLL在极端碱性pH下表现出更高的活性。TerBLL在2-8M尿素中保留了50%的活性，而TerBSL为13%。对TerBLL特性的研究表明，它具有抗伤害感受作用，在热板试验中可减轻腹痛并延长潜伏期，可能是通过类似于吗啡的μ-阿片受体阻断作用。TerBLL在0.25和1 mg/kg的剂量下表现出抗溃疡活性，可将溃疡形成减少多达33%，与泮托拉唑（80 mg/kg）相当。TerBLL的理化特性，除了其止痛和胃保护作用外，还突出了其治疗前景，这与其传统用途一致。
