Liao Jian-You, Yang Bing, Shi Chuan-Ping, Deng Wei-Xi, Deng Jin-Si, Cen Mei-Feng, Zheng Bing-Qi, Zhan Zi-Ling, Liang Qiao-Ling, Wang Ji-En, Tao Shuang, Lu Daning, Liang Maojin, Zhang Yu-Chan, Yin Dong
Department of Medical Research Center, Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Guangdong-Hong Kong Joint Laboratory for RNA Medicine, Sun Yat-Sen Memorial Hospital, Sun Yat-sen University, 107 Yan Jiang West Road, Guangzhou, Guangdong 510120, China.
Department of Precision Medicine Center, Shenshan Central Hospital, Sun Yat-Sen Memorial Hospital, Sun Yat-sen University, 1 Heng Er Road, Dongyong Town, Shanwei, Guangdong 516621, China.
Nucleic Acids Res. 2025 Jan 6;53(D1):D220-D232. doi: 10.1093/nar/gkae1028.
RNA-binding proteins (RBPs) play key roles in a wide range of physiological and pathological processes. To facilitate the investigation of RBP functions and disease associations, we updated the EuRBPDB and renamed it as RBPWorld (http://research.gzsys.org.cn/rbpworld/#/home). Leveraging 998 RNA-binding domains (RBDs) and 87 RNA-binding Proteome (RBPome) datasets, we successfully identified 1 393 413 RBPs from 445 species, including 3030 human RBPs (hRBPs). RBPWorld includes primary RNA targets of diverse hRBPs, as well as potential downstream regulatory pathways and alternative splicing patterns governed by various hRBPs. These insights were derived from analyses of 1515 crosslinking immunoprecipitation-seq datasets and 616 RNA-seq datasets from cells with hRBP gene knockdown or knockout. Furthermore, we systematically identified 929 RBPs with multi-functions, including acting as metabolic enzymes and transcription factors. RBPWorld includes 838 disease-associated hRBPs and 970 hRBPs that interact with 12 disease-causing RNA viruses. This provision allows users to explore the regulatory roles of hRBPs within the context of diseases. Finally, we developed an intuitive interface for RBPWorld, facilitating users easily access all the included data. We believe that RBPWorld will be a valuable resource in advancing our understanding of the biological roles of RBPs across different species.
RNA结合蛋白（RBPs）在广泛的生理和病理过程中发挥关键作用。为了便于研究RBPs的功能及其与疾病的关联，我们对EuRBPDB进行了更新，并将其重新命名为RBPWorld（http://research.gzsys.org.cn/rbpworld/#/home）。利用998个RNA结合结构域（RBDs）和87个RNA结合蛋白质组（RBPome）数据集，我们成功地从445个物种中鉴定出1393413个RBPs，其中包括3030个人类RBPs（hRBPs）。RBPWorld包含了多种hRBPs的主要RNA靶点，以及由各种hRBPs调控的潜在下游调控途径和可变剪接模式。这些见解来自于对1515个交联免疫沉淀测序数据集和616个来自hRBP基因敲低或敲除细胞的RNA测序数据集的分析。此外，我们系统地鉴定出929个具有多种功能的RBPs，包括作为代谢酶和转录因子。RBPWorld包含838个与疾病相关的hRBPs和970个与12种致病RNA病毒相互作用的hRBPs。这一功能使用户能够在疾病背景下探索hRBPs的调控作用。最后，我们为RBPWorld开发了一个直观的界面，方便用户轻松访问所有包含的数据。我们相信，RBPWorld将成为推动我们理解不同物种中RBPs生物学作用的宝贵资源。
