Interdisciplinary Unit: Lipids, Analytical and Biological Systems Lip(Sys)2, Faculty of Pharmacy, Université Paris-Saclay, F-91400 Orsay, France.
Laboratoire d'étude cinétique en milieu hétérogène (LECH), Saint Joseph University, Campus des Sciences et Technologies, Mar Roukos, Mkallès, P.O Box 11-514, Riad El Solh, 1107 2050 Beirut, Lebanon.
Analyst. 2024 Nov 18;149(23):5693-5703. doi: 10.1039/d4an01244e.
Skin health relies heavily on a well-maintained cutaneous barrier. While the detrimental effects of UV radiation on the epidermis are established, the impact of blue light, a significant component of sunlight and artificial sources, is less clear. This study aims to explore blue light's influence on the reconstructed human epidermis (RHE) using two complementary analytical approaches: Raman microspectroscopy and normal phase liquid chromatography coupled with high-resolution mass spectrometry (NPLC/HR-MS). RHE samples were exposed to blue light (415 nm and 455 nm) during different stages of their maturation. Raman spectra were acquired for both irradiated and non-irradiated (control) samples. Raman descriptors were analyzed to assess potential alterations in the structural organization of proteins and lipids' conformational changes. Additionally, lipids from RHE samples were extracted and analyzed using NPLC/HR-MS. Blue light exposure led to changes in the structural organization of RHE lipids and proteins, as well as changes in the lipid composition. These changes varied depending on the wavelength and exposure dose. Exposure to blue light could disrupt the integrity of the skin's protective barrier, leading to increased sensitivity to environmental stressors and potential skin damage.
皮肤健康在很大程度上依赖于良好维持的皮肤屏障。虽然紫外线辐射对表皮的有害影响已得到证实，但阳光和人工光源中的重要组成部分——蓝光的影响则不太明确。本研究旨在使用两种互补的分析方法——拉曼微光谱和正相液相色谱与高分辨率质谱联用（NPLC/HR-MS）来探索蓝光对重建的人类表皮（RHE）的影响。在 RHE 成熟的不同阶段，用蓝光（415nm 和 455nm）照射 RHE 样本。对辐照和非辐照（对照）样本（即未暴露于蓝光的样本）进行拉曼光谱采集。分析拉曼描述符以评估蛋白质结构组织和脂质构象变化的潜在改变。此外，还使用 NPLC/HR-MS 从 RHE 样本中提取和分析脂质。蓝光照射导致 RHE 脂质和蛋白质的结构组织发生改变，脂质组成也发生变化。这些变化取决于波长和暴露剂量。蓝光照射可能会破坏皮肤保护屏障的完整性，导致对环境应激源的敏感性增加，并可能导致皮肤损伤。
