Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan.
Methods Mol Biol. 2025;2869:29-39. doi: 10.1007/978-1-0716-4204-7_5.
The adaptability of plants to given environmental conditions is achieved through the regulation of various genes. The functional regulation of genes depends on precise transcriptional and translational controls that, at the end, determine the expression of the translated products (proteins). Here, I describe the immunoblotting method for the detection of proteins expressed in rice leaves. This method includes protein extraction, protein separation, electroblotting, and immune detection.
植物对特定环境条件的适应性是通过调节各种基因来实现的。基因的功能调节取决于精确的转录和翻译控制，最终决定翻译产物（蛋白质）的表达。在这里，我描述了用于检测水稻叶片中表达的蛋白质的免疫印迹法。该方法包括蛋白质提取、蛋白质分离、电转移和免疫检测。
