原位杂交的基因表达空间分析。

Spatial Analysis of Gene Expression by In Situ Hybridization.

机构信息

National Institute of Genetics, Mishima, Shizuoka, Japan.

Graduate School of Agricultural Regional Vitalization, Kibi International University, Minamiawaji, Japan.

出版信息

Methods Mol Biol. 2025;2869:49-59. doi: 10.1007/978-1-0716-4204-7_7.

DOI:10.1007/978-1-0716-4204-7_7

Abstract

The analysis of the spatial expression patterns of genes is important for deciphering their functions. In situ hybridization provides insight into gene expression patterns at the cellular level. Here we describe a procedure for performing in situ hybridization on sections of paraffin-embedded tissue, including synthesis of labeled RNA probes, hybridization of the probes with target mRNAs, and immunological detection of the signals. The method enables evaluation of the expression patterns of genes in a variety of tissues of rice.

摘要

基因的空间表达模式分析对于破译其功能非常重要。原位杂交技术可深入了解细胞水平的基因表达模式。本文描述了一种对石蜡包埋组织切片进行原位杂交的方法，包括标记 RNA 探针的合成、探针与靶 mRNA 的杂交以及信号的免疫检测。该方法可用于评估水稻多种组织中基因的表达模式。

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