Faculty of Agriculture, Ryukoku University, Shiga, Japan.
Institute for Advanced Biosciences, Keio University, Yamagata, Japan.
Methods Mol Biol. 2025;2869:123-134. doi: 10.1007/978-1-0716-4204-7_13.
Lasy-Seq (low-cost and easy RNA-seq) is a high-throughput library preparation method for 3' RNA-seq. Usage of oligo(dT) primers with unique index sequences allows simultaneous reverse transcription (RT) reaction and sample indexing. Therefore, each post-RT step, which was performed on a sample-by-sample basis in a conventional method, can be performed on a large number of samples together in a single tube. With the Lasy-Seq, the sequencing-ready library pool can be prepared from a few to several hundred samples in a few days.
Lasy-Seq（低成本、易操作的 RNA-seq 方法）是一种高通量的 3' RNA-seq 文库制备方法。使用带有独特索引序列的寡聚（dT）引物可以同时进行逆转录（RT）反应和样本标记。因此，在传统方法中每个样本单独进行的 RT 后步骤，现在可以在一个管中同时对大量样本进行。使用 Lasy-Seq，可在数天内从几个到几百个样本中制备测序就绪的文库池。
