Mamedov Arslan, Rumbinaitė Eglė, Romann Sebastian, Verikas Dovydas, Jakuška Povilas, Aitaliyev Serik, Benetis Rimantas, Stankevičius Edgaras
Department of Cardiac, Thoracic and Vascular Surgery, Faculty of Medicine, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
Department of Cardiology, of Medicine, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
Gen Thorac Cardiovasc Surg. 2024 Nov 5. doi: 10.1007/s11748-024-02097-9.
Minor defects in the mitochondrial ATP-generating system and post-cardioplegia oxidative phosphorylation can negatively impact cardiac function in immature hearts. This study aimed to examine the mitochondrial respiratory pathway using three different cardioplegic solutions (Custodiol HTK, St. Thomas, and Del Nido) during moderate (1 h) and long (3 h) ischemic periods.
A total of 41 male Wistar albino rats were utilized in this study. Five experiments were conducted without the use of any cardioplegic solution (CP0 group). To assess both moderate and prolonged ischemic periods, six experiments were carried out in each of the following groups: CP1 group (St. Thomas solution), CP2 group (Custodiol HTK solution), and CP3 group (Del Nido solution).
After 1 h, the highest mitochondrial respiration rate was observed in the CP3 group and the lowest in the CP1 group (p = 0.006). After adding ADP substrate, the highest mitochondrial ATP-production-coupled respiration was recorded in the CP3 group, which was similar to the control group CP0. After 3 h, while evaluating the ratio between mitochondrial respiration ATP-production coupled and basal respiration, significant differences were found between CP1 group and CP3 group (p = 0.035), as well as between the CP1 and CP0 groups (p = 0.045). Additionally, by assessing the condition of the outer mitochondrial membrane using the Cyt C effect (Cyt/Phos [ADP]), significant differences were observed between the CP1 and CP3 group (p = 0.004), as well as between CP1 and CP0 groups (p = 0.003).
Del Nido cardioplegic solution provided optimal mitochondrial protection under moderate and long myocardial ischemia conditions.
线粒体ATP生成系统的微小缺陷以及心脏停搏后氧化磷酸化过程会对未成熟心脏的心脏功能产生负面影响。本研究旨在使用三种不同的心脏停搏液（HTK液、圣托马斯液和德尔尼多液），在中度（1小时）和长时间（3小时）缺血期检测线粒体呼吸途径。
本研究共使用了41只雄性Wistar白化大鼠。进行了5次实验，未使用任何心脏停搏液（CP0组）。为评估中度和长时间缺血期，在以下每组中进行了6次实验：CP1组（圣托马斯液）、CP2组（HTK液）和CP3组（德尔尼多液）。
1小时后，CP3组的线粒体呼吸率最高，CP1组最低（p = 0.006）。添加ADP底物后，CP3组的线粒体ATP生成偶联呼吸率最高，与对照组CP0相似。3小时后，在评估线粒体呼吸ATP生成偶联与基础呼吸的比率时，CP1组和CP3组之间存在显著差异（p = 0.035），CP1组和CP0组之间也存在显著差异（p = 0.045）。此外，通过使用细胞色素C效应（Cyt/Phos [ADP]）评估线粒体外膜的状况，CP1组和CP3组之间存在显著差异（p = 0.004），CP1组和CP0组之间也存在显著差异（p = 0.003）。
在中度和长时间心肌缺血条件下，德尔尼多心脏停搏液提供了最佳的线粒体保护。
