P Amruth, M Rosemol Jacob, Joy Jean Mary, Visnuvinayagam S, Remya S, Mathew Suseela
Biochemistry and Nutrition Division, ICAR-Central Institute of Fisheries Technology, Cochin 682029, Kerala, India; Faculty of Marine Sciences, Cochin University of Science and Technology, Cochin 682022, Kerala, India; Department of Life Sciences, Christ University, Hosur Main Road, Bhavani Nagar, Bangalore 560029, Karnataka, India.
Biochemistry and Nutrition Division, ICAR-Central Institute of Fisheries Technology, Cochin 682029, Kerala, India; Faculty of Marine Sciences, Cochin University of Science and Technology, Cochin 682022, Kerala, India.
Int J Biol Macromol. 2024 Dec;282(Pt 5):137084. doi: 10.1016/j.ijbiomac.2024.137084. Epub 2024 Nov 3.
Wound healing remains a critical challenge in healthcare, requiring advanced wound dressings with superior properties like transparency, absorbency, and biocompatibility. However, gaps exist in the use of marine-derived biopolymers for sustainable dressings. This study addresses this gap by combining κ-carrageenan (KC) with polyvinyl pyrrolidone (PVP) to develop transparent and absorbent biodegradable films through solvent casting and lyophilization techniques. Lyophilized films exhibited superior absorbency (9.17 g/cm) and moisture management, with a water vapour transmission rate of 3990.67 g/m/24 h, while solvent-cast films showed 78 % transmittance, enabling wound visualization. Mechanical testing revealed high tensile strength (31.5 MPa) and folding endurance (410 folds), ensuring durability. In vitro bactericidal assays confirmed efficacy against MRSA and E. coli, and in vivo tests on Wistar rats showed complete wound healing within 16 days with 91.1 % closure, outperforming untreated controls (76.7 %). This is the first study to explore lyophilized KC-PVP films for wound dressing applications, demonstrating potential for drug release, absorbency, and biodegradability. The innovative combination of biopolymers and fabrication techniques offers a sustainable, high-performance solution for wound care.
伤口愈合仍然是医疗保健领域的一项严峻挑战，需要具有透明度、吸水性和生物相容性等卓越性能的先进伤口敷料。然而，在将海洋来源的生物聚合物用于可持续敷料方面仍存在差距。本研究通过将κ-卡拉胶（KC）与聚乙烯吡咯烷酮（PVP）相结合，采用溶剂浇铸和冻干技术开发出透明且具有吸水性的可生物降解薄膜，以填补这一差距。冻干薄膜表现出卓越的吸水性（9.17 g/cm）和水分管理能力，水蒸气透过率为3990.67 g/m²/24 h，而溶剂浇铸薄膜的透光率为78%，能够实现伤口可视化。力学测试显示出高拉伸强度（31.5 MPa）和耐折性（410次折叠），确保了耐用性。体外杀菌试验证实了对耐甲氧西林金黄色葡萄球菌和大肠杆菌的有效性，对Wistar大鼠的体内试验表明，在16天内伤口完全愈合，愈合率为91.1%，优于未处理的对照组（76.7%）。这是第一项探索冻干KC-PVP薄膜用于伤口敷料应用的研究，证明了其在药物释放、吸水性和生物降解性方面的潜力。生物聚合物与制造技术的创新结合为伤口护理提供了一种可持续的高性能解决方案。
