Gelinas D, Callard G V
Department of Biology, Boston University, MA 02215, USA.
J Neuroendocrinol. 1993 Dec;5(6):635-41. doi: 10.1111/j.1365-2826.1993.tb00533.x.
Using an animal model in which neural aromatase is apparently overexpressed (the goldfish, Carassius auratus) and an anti-human placental antibody which specifically crossreacts with goldfish brain aromatase, aromatase-immunoreactive neuronal cell bodies and fibers have been localized within the retina. These include a subset of horizontal cells, bipolar cells, and amacrine cells of the inner nuclear layer, some fibers of the outer and inner synaptic layers and certain cells of the ganglion cell layer; photoreceptors were never labeled. Some ganglion cell projections to the brain via the optic nerve and optic tract were aromatase-positive, as were small neurons of the stratum periventriculare (SPV) and fibers of two other strata of the optic tectum. Aromatase activity, as measured by [3H]androgen by tissue homogenates and cell cultures, confirmed the presence of aromatase in retina and in brain regions containing the optic tectum. This localization of the rate-limiting enzyme in estrogen biosynthesis suggests that neuroestrogen derived from circulating androgen m ay modulate transmission and integration of visual information important for reproduction in this species.
利用一种神经芳香化酶明显过度表达的动物模型（金鱼，Carassius auratus）以及一种能与金鱼脑芳香化酶特异性交叉反应的抗人胎盘抗体，已将芳香化酶免疫反应性神经元细胞体和纤维定位在视网膜内。这些包括内核层的水平细胞、双极细胞和无长突细胞的一个亚群、外突触层和内突触层的一些纤维以及神经节细胞层的某些细胞；光感受器从未被标记。一些通过视神经和视束向脑投射的神经节细胞是芳香化酶阳性的，脑室周围层（SPV）的小神经元以及视顶盖其他两层的纤维也是如此。通过组织匀浆和细胞培养用[3H]雄激素测定的芳香化酶活性，证实了视网膜和含有视顶盖的脑区中存在芳香化酶。这种雌激素生物合成限速酶的定位表明，循环雄激素衍生的神经雌激素可能调节该物种中对繁殖重要的视觉信息的传递和整合。
