Metto Melaku, Tesfaye Alemu, Atlabachew Minaleshewa, Abebe Atakilt
Department of Chemistry, College of Science, Bahir Dar University, Ethiopia.
Department of Chemistry, College of Natural and Computational Sciences, Injibara University, Ethiopia.
Heliyon. 2024 Oct 10;10(20):e38794. doi: 10.1016/j.heliyon.2024.e38794. eCollection 2024 Oct 30.
A Novel bioanalytical detector based on Poly(Diaquabis(1,10-phenanthroline copper (II) Iodide) (poly([Cu(HO)P]I)/GCE) has been fabricated by potentiodynamic polymerization of [Cu(HO)P]I. The synthesized complex and the fabricated electrodes showed a promising electrocatalytic behavior towards the electrooxidation of Trimethoprim (TPM). Based on the regression coefficient value of scan rate with peak current and square root of peak current, the oxidation of TMP at poly([Cu(HO)P]I)/GCE was dominated by diffusion. Under the optimized experimental conditions and square wave voltammetric parameters, the poly([Cu(HO)P]I)/GCE revealed very wide responses in the range of concentration of 500 nM-160 μM with a limit of detection of 3.91 nM and a Limit of quantification of 13.02 nM with the relative standard deviation below 1.75 %. The valuable applicability of the fabricated sensor for the determination of TMP in real samples, including cow's milk, blood serum, and human urine, was successfully investigated. The electroanalysis results from the spike recovery and interference study provided an excellent range of recovery with an error percent of below 3.0 % in the combination effect with the potential interferents. The sensor exhibited excellent reproducibility, sensitivity, long-term stability, high accuracy, and fast response. The developed bioanalytical sensor revealed the best application prospect for biomedicine and environmental monitoring activities.
基于聚（二水双（1,10 - 菲咯啉铜（II）碘化物）（聚（[Cu(HO)P]I）/玻碳电极）的新型生物分析检测器通过[Cu(HO)P]I的恒电位聚合制备而成。合成的配合物和制备的电极对甲氧苄啶（TPM）的电氧化表现出良好的电催化行为。基于扫描速率与峰电流以及峰电流平方根的回归系数值，TPM在聚（[Cu(HO)P]I）/玻碳电极上的氧化受扩散控制。在优化的实验条件和方波伏安参数下，聚（[Cu(HO)P]I）/玻碳电极在500 nM - 160 μM的浓度范围内显示出非常宽的响应，检测限为3.91 nM，定量限为13.02 nM，相对标准偏差低于1.75%。成功研究了所制备传感器在实际样品（包括牛奶、血清和人尿）中测定TPM的宝贵适用性。加标回收率和干扰研究的电分析结果在与潜在干扰物的联合效应中提供了优异的回收率范围，误差百分比低于3.0%。该传感器表现出优异的重现性、灵敏度、长期稳定性、高准确性和快速响应。所开发的生物分析传感器在生物医学和环境监测活动中显示出最佳的应用前景。
