Xing Gaowa, Lin Jin-Ming
Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China.
Biomicrofluidics. 2024 Nov 4;18(6):061301. doi: 10.1063/5.0231916. eCollection 2024 Dec.
Food security related to bacterial pathogens has seriously threatened human life and caused public health problems. Most of the reported methods are targeted at known major pathogens commonly found in food samples, but to some extent, they have the disadvantage of lacking simplicity, speed, high throughput, and high sensitivity. Microfluidics has become a promising tool for foodborne bacteria analysis and addresses the above limitations. In this perspective, we briefly discussed the ongoing research and development in this field. We outline the major types of microfluidics, the strategies of target biorecognition, and signal amplification technologies in the microfluidic system for the foodborne bacteria analysis. We also proposed the future directions of microfluidics for foodborne bacterial analysis, which aims to integrate multiple technologies toward intelligent analysis with high selectivity and sensitivity for unknown samples, multiple bacterial detection, and simultaneous detection of multiple food pollutants.
与细菌病原体相关的食品安全已严重威胁到人类生命并引发了公共卫生问题。大多数已报道的方法针对的是食品样本中常见的已知主要病原体,但在一定程度上,它们存在缺乏简便性、速度、高通量和高灵敏度的缺点。微流控技术已成为用于食源细菌分析的一种有前景的工具,并解决了上述局限性。从这个角度出发,我们简要讨论了该领域正在进行的研究与开发。我们概述了微流控技术的主要类型、目标生物识别策略以及微流控系统中用于食源细菌分析的信号放大技术。我们还提出了微流控技术用于食源细菌分析的未来发展方向,旨在整合多种技术以实现对未知样本的高选择性和高灵敏度智能分析、多种细菌检测以及多种食品污染物的同时检测。