Zhao Xihong, Li Mei, Liu Yao
Research Center for Environmental Ecology and Engineering, Key Laboratory for Green Chemical Process of Ministry of Education, Key Laboratory for Hubei Novel Reactor & Green Chemical Technology, School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan 430205, China.
School of Pharmacy and Food Science, Zhuhai College of Jilin University, Zhuhai 519041, China.
Microorganisms. 2019 Sep 23;7(10):381. doi: 10.3390/microorganisms7100381.
Food safety is of obvious importance, but there are frequent problems caused by foodborne pathogens that threaten the safety and health of human beings worldwide. Although the most classic method for detecting bacteria is the plate counting method, it takes almost three to seven days to get the bacterial results for the detection. Additionally, there are many existing technologies for accurate determination of pathogens, such as polymerase chain reaction (PCR), enzyme linked immunosorbent assay (ELISA), or loop-mediated isothermal amplification (LAMP), but they are not suitable for timely and rapid on-site detection due to time-consuming pretreatment, complex operations and false positive results. Therefore, an urgent goal remains to determine how to quickly and effectively prevent and control the occurrence of foodborne diseases that are harmful to humans. As an alternative, microfluidic devices with miniaturization, portability and low cost have been introduced for pathogen detection. In particular, the use of microfluidic technologies is a promising direction of research for this purpose. Herein, this article systematically reviews the use of microfluidic technology for the rapid and sensitive detection of foodborne pathogens. First, microfluidic technology is introduced, including the basic concepts, background, and the pros and cons of different starting materials for specific applications. Next, the applications and problems of microfluidics for the detection of pathogens are discussed. The current status and different applications of microfluidic-based technologies to distinguish and identify foodborne pathogens are described in detail. Finally, future trends of microfluidics in food safety are discussed to provide the necessary foundation for future research efforts.
食品安全至关重要，但食源性病原体引发的问题频发，威胁着全球人类的安全与健康。虽然检测细菌最经典的方法是平板计数法，但检测细菌结果几乎需要三到七天。此外，现有许多准确测定病原体的技术，如聚合酶链反应（PCR）、酶联免疫吸附测定（ELISA）或环介导等温扩增（LAMP），但由于预处理耗时、操作复杂和假阳性结果，它们不适用于及时快速的现场检测。因此，如何快速有效地预防和控制对人类有害的食源性疾病的发生仍是一个紧迫的目标。作为一种替代方法，具有小型化、便携性和低成本的微流控装置已被引入用于病原体检测。特别是，微流控技术的应用是实现这一目标的一个有前途的研究方向。在此，本文系统综述了微流控技术在食源性病原体快速灵敏检测中的应用。首先，介绍了微流控技术，包括基本概念、背景以及特定应用中不同起始材料的优缺点。接下来，讨论了微流控技术在病原体检测中的应用和问题。详细描述了基于微流控的技术区分和鉴定食源性病原体的现状及不同应用。最后，讨论了微流控技术在食品安全方面的未来趋势，为未来的研究工作提供必要的基础。
