Das Upma, Gautam Vanita, Shubham Snigdha, Raut Shristi
Department of Conservative Dentistry and Endodontics, Universal College of Medical Sciences, Bhairahawa, Nepal.
Department of Microbiology, Universal College of Medical Sciences, Bhairahawa, Nepal.
Int J Biomater. 2024 Oct 29;2024:8867854. doi: 10.1155/2024/8867854. eCollection 2024.
The absence of a barrier in an open root apex makes endodontic treatment challenging as root-filling material can easily reach the surrounding tissue. The aim of the study was to compare the apical microleakage associated with mineral trioxide aggregate (MTA), biodentine, custom-made gutta-percha with MTA plus and custom-made gutta-percha with Adseal in immature permanent teeth by dye penetration method. Apical 2 mm of 60 single-rooted mandibular premolar teeth was resected to create divergent open apices and 10 teeth each were filled with Biodentine plug, MTA plug, custom-made gutta-percha with MTA plus sealer and custom-made gutta-percha with Adseal sealer. Ten teeth each acted as positive and negative controls. All the samples were stored at 37°C at 100% humidity for 5 weeks and then immersed in 2% Rhodamine B dye for 24 h. Transverse sectioning was done apically at 1 mm and 3 mm to evaluate dye penetration under a fluorescence microscope using ImageJ software. There was a significantly greater microleakage at 1 mm cross section compared to 3 mm ( < 0.0001). At 1 mm cross section, the apical microleakage was the highest for the MTA plug with a mean leakage percentage of 48.08 ± 16.38, a mean depth of leakage of 0.46 ± 0.10 mm and a mean area of leakage of 1.35 ± 0.74 mm, compared to other groups, and the difference was statistically significant. However, at a 3 mm cross section, MTA plus sealer with gutta-percha demonstrated the highest mean leakage percentage (25.01 ± 7.77) compared to other groups and the difference was statistically significant ( = 0.03). It can be concluded that the 3-mm-thick apical plug provided better sealing of the open apex compared to the 1 mm apical plug and there was no significant difference in microleakage among the Biodentine plug, MTA plug and Adseal sealer with gutta-percha plug at 3 mm cross section.
开放根尖孔缺乏屏障使得根管治疗具有挑战性，因为根管充填材料很容易到达周围组织。本研究的目的是通过染料渗透法比较在未成熟恒牙中，与矿物三氧化物凝聚体（MTA）、生物陶瓷、含MTA Plus的定制牙胶以及含Adseal的定制牙胶相关的根尖微渗漏情况。将60颗单根下颌前磨牙的根尖2毫米切除，以形成发散的开放根尖孔，每组10颗牙齿分别用生物陶瓷充填、MTA充填、含MTA Plus封闭剂的定制牙胶充填以及含Adseal封闭剂的定制牙胶充填。每组10颗牙齿分别作为阳性和阴性对照。所有样本在37℃、湿度100%的条件下保存5周，然后浸入2%的罗丹明B染料中24小时。在根尖1毫米和3毫米处进行横向切片，使用ImageJ软件在荧光显微镜下评估染料渗透情况。与3毫米处相比，1毫米横截面处的微渗漏明显更大（<0.0001）。在1毫米横截面处，MTA充填组的根尖微渗漏最高，平均渗漏百分比为48.08±16.38，平均渗漏深度为0.46±0.10毫米，平均渗漏面积为1.35±0.74毫米，与其他组相比，差异具有统计学意义。然而，在3毫米横截面处，含MTA Plus封闭剂的牙胶组的平均渗漏百分比最高（25.01±7.77），与其他组相比，差异具有统计学意义（=0.03）。可以得出结论，与1毫米根尖充填物相比，3毫米厚的根尖充填物能更好地封闭开放根尖孔，在3毫米横截面处，生物陶瓷充填、MTA充填以及含Adseal封闭剂的牙胶充填之间的微渗漏没有显著差异。
