锌释放型玻璃离子水门汀的 MMP 抑制作用、边缘完整性和细胞毒性。

MMP inhibition, marginal integrity and cytotoxicity of zinc-releasing GIC.

机构信息

Department of Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.

Department of Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan; Department of Operative Dentistry, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.

出版信息

J Dent. 2024 Dec;151:105450. doi: 10.1016/j.jdent.2024.105450. Epub 2024 Nov 4.

DOI:10.1016/j.jdent.2024.105450

PMID:39505291

Abstract

OBJECTIVES

The degradation of the restorative-dentin interface due to endogenous dentin enzymes, such as matrix metalloproteinases (MMPs), is a significant issue that accelerates the deterioration of the dentin matrix and leads to the failure of restorative treatments. Caredyne Restore (CR), a novel glass ionomer cement (GIC) with zinc ions in its formulation, represents the latest effort to mitigate this issue. This investigation aimed to evaluate the MMPs inhibitory effect, marginal integrity, and cytotoxicity of CR compared to a conventional GIC, Fuji IX (FIX).

METHODS

The inhibitory effect of CR on MMPs activity was evaluated using in-situ zymography to visualize the endogenous gelatinolytic activity in the GIC-dentin interface. Additionally, CR's sealing properties were investigated using dye-assisted confocal laser microscopy (CLSM) to assess marginal leakage across the GIC-dentin interface. Both in-situ zymography and CLSM observations were conducted 1 day and seven days after pH cycling. Finally, the cytotoxicity of the GIC eluates was examined on rat dental pulp cells (RPCC2A). Assay measurements were performed after 24 and 48 h of incubation with test solutions prepared using various GIC eluate concentrations.

RESULTS

The MMPs activity of the CR specimens was significantly lower than that of FIX specimens after seven days of pH cycling. Signs of marginal leakage were also lower in the CR specimens. Comparison of CR and FIX eluates at the same concentration showed no significant difference in terms of biocompatibility.

CONCLUSIONS

Caredyne restore is capable of inhibiting endogenous enzyme activity and improving sealing properties, while maintaining sufficient biocompatibility.

CLINICAL SIGNIFICANCE

Zinc ions released from Caredyne Restore offer a safe way to improve the quality of dental restorations and promote minimally invasive treatment, especially in lesions where the dentin matrix is susceptible to enzymatic degradation and recurrent caries.

摘要

目的

内源性牙本质酶（如基质金属蛋白酶 [MMPs]）导致的修复-牙本质界面降解是一个重大问题，它加速了牙本质基质的恶化，并导致修复治疗失败。Caredyne Restore（CR）是一种新型玻璃离子水门汀（GIC），其配方中含有锌离子，是缓解这一问题的最新尝试。本研究旨在评估 CR 对 MMPs 活性的抑制作用、边缘完整性和细胞毒性，并与传统 GIC Fuji IX（FIX）进行比较。

方法

通过原位酶谱法可视化 GIC-牙本质界面内源性明胶酶活性，评估 CR 对 MMPs 活性的抑制作用。此外，还通过染色辅助共聚焦激光显微镜（CLSM）评估 CR 的密封性能，以评估 GIC-牙本质界面的边缘渗漏情况。原位酶谱法和 CLSM 观察均在 pH 循环后 1 天和 7 天进行。最后，通过大鼠牙髓细胞（RPCC2A）检测 GIC 浸提液的细胞毒性。使用不同 GIC 浸提液浓度制备的测试溶液孵育 24 和 48 小时后，进行测定。