Pukou Hospital of Chinese Medicine affiliated to China Pharmaceutical University, China Pharmaceutical University, 639 Longmian road, Nanjing, China.
Sci Rep. 2024 Nov 6;14(1):27014. doi: 10.1038/s41598-024-78364-9.
Ermiao wan (EMW) is a classical traditional Chinese medicine formula, with two modified versions including Sanmiao wan (SMW) and Simiao wan (FMW). These Ermiao wan categorized formulas (ECFs) are traditionally used to treat gouty arthritis and hyperuricemia. However, their potential benefits and mechanisms on diabetes remain to be explored. This study aims to investigate the overall effects and biological differences of ECFs in high fat diet (HFD)-fed mice based on network pharmacology and transcriptomics. ECFs significantly reduced body weight, improved oral glucose tolerance, decreased fat accumulation, and lowered serum insulin and inflammatory cytokine levels in HFD-fed mice. FMW had better efficacy than EMW and SMW. Network pharmacology analysis revealed that ECFs targeted functional modules associated with chronic inflammation, lipid metabolism, and glucose metabolism. Transcriptome results also showed ECFs could inhibit genes associated with inflammation and upregulated some genes in lipid metabolism. Comprehensive analysis and QPCR verification indicated the beneficial effects of ECFs on diabetes might be attributed to the regulation of Ddit3, Ccl2, Esr1, and Cyp7a1. This study provides a theoretical basis for the clinical use of ECFs.
二妙丸（EMW）、三妙丸（SMW）和四妙丸（FMW）是经典的中药方剂，均由苍术、黄柏组成。这些二妙丸类方（ECFs）传统上用于治疗痛风性关节炎和高尿酸血症。然而，它们在糖尿病方面的潜在益处和机制仍有待探索。本研究旨在基于网络药理学和转录组学研究高脂饮食（HFD）喂养的小鼠中 ECFs 的整体作用和生物学差异。研究结果表明，ECFs 可显著降低 HFD 喂养小鼠的体重、改善口服葡萄糖耐量、减少脂肪堆积、降低血清胰岛素和炎症细胞因子水平。与 EMW 和 SMW 相比，FMW 的疗效更好。网络药理学分析显示，ECFs 靶向与慢性炎症、脂质代谢和葡萄糖代谢相关的功能模块。转录组结果还表明，ECFs 可以抑制与炎症相关的基因，并上调某些脂质代谢相关基因。综合分析和 QPCR 验证表明，ECFs 对糖尿病的有益作用可能归因于对 Ddit3、Ccl2、Esr1 和 Cyp7a1 的调节。本研究为 ECFs 的临床应用提供了理论依据。
