评价快速多重聚合酶链反应检测对脑膜炎和脑炎诊断的影响：一项回顾性分析。
Evaluating the Impact of Rapid Multiplex PCR Testing on Meningitis and Encephalitis Diagnosis: a Retrospective Analysis.
出版信息
Clin Lab. 2024 Nov 1;70(11). doi: 10.7754/Clin.Lab.2024.240612.
BACKGROUND
Meningitis and encephalitis are life-threatening neurological emergencies requiring prompt diagnosis and treatment. Traditional diagnostic methods often lack the speed and sensitivity needed for rapid clinical decision-making. This study evaluates the effectiveness of rapid multiplex PCR testing in diagnosing meningitis and encephalitis.
METHODS
Between March 28, 2022, and May 31, 2024, we analyzed cerebrospinal fluid collected from a cohort of patients aged 24 to 87 at Asia University Hospital. These specimens were analyzed using the FilmArray Meningitis/Encephalitis (ME) Panel.
RESULTS
The study included 19 patients who underwent FilmArray ME panel testing. Of these, 2 (10.5%) tested positive, while 17 (89.5%) tested negative. Among the positive specimens, herpes simplex virus-1 was detected in one patient, and Varicella zoster virus in the other.
CONCLUSIONS
This study underscores the clinical value of the FilmArray ME panel as a rapid and reliable tool for diagnosing meningitis and encephalitis. Multiplex PCR significantly improves diagnostic accuracy and reduces time to diagnosis, facilitating faster clinical intervention and improved patient outcomes.
背景
脑膜炎和脑炎是危及生命的神经系统急症，需要及时诊断和治疗。传统的诊断方法往往缺乏快速临床决策所需的速度和灵敏度。本研究评估了快速多重聚合酶链反应（PCR）检测在诊断脑膜炎和脑炎中的效果。
方法
2022 年 3 月 28 日至 2024 年 5 月 31 日，我们分析了亚洲大学医院 24 至 87 岁患者的脑脊液。使用 FilmArray 脑膜炎/脑炎（ME）检测试剂盒进行分析。
结果
本研究纳入了 19 名接受 FilmArray ME 检测试剂盒检测的患者。其中，2 名（10.5%）检测呈阳性，17 名（89.5%）检测呈阴性。在阳性标本中，1 例为单纯疱疹病毒-1 型，另 1 例为水痘带状疱疹病毒。
结论
本研究强调了 FilmArray ME 检测试剂盒作为一种快速、可靠的脑膜炎和脑炎诊断工具的临床价值。多重 PCR 显著提高了诊断准确性，并缩短了诊断时间，有助于更快的临床干预和改善患者结局。
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