Albrahim Tarfa, Alangry Raghad, Alotaibi Raghad, Almandil Leen, Alburikan Sara, Aloudah Hisham S, Alahmed Mohammed, Alonazi Mona
Department of Health Sciences, Clinical Nutrition, College of Health and Rehabilitation Sciences, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia.
Prince Naif for Health Research Center, King Saud University, Riyadh, Saudi Arabia.
Front Physiol. 2024 Oct 23;15:1403772. doi: 10.3389/fphys.2024.1403772. eCollection 2024.
Osteoporosis is a chronic bone metabolic disease that affects millions of people worldwide, particularly the elderly and postmenopausal women. It is characterized by weakened bones, increasing the risk of fractures and leading to significant morbidity and mortality. The goal of the current study is to examine the reported osteo-preservative effects of exercise and/or fasting in the Ramadan fasting model (RFM) in ovariectomized (OVX) rats.
The experimental intervention started 1 month following the ovariectomy procedure and consisted of five 15-min exercise sessions per week at 18-25 m/min and/or an approximately 13-h fast from sunrise to sunset (6:00 AM-19:00 PM). Serum bone metabolism biomarker levels were measured, and mineral concentrations in femoral ashed bones and digested serum were determined. Additionally, serum bone alkaline phosphatase (b-ALP), parathyroid hormone, osteocalcin, calcitonin, and vitamin D3 concentrations were measured using the competitive enzyme immunoassay technique.
Calcium, magnesium, and phosphorus showed a notable decrease in mineral concentration among OVX rat femurs compared with the combination group (OVX + RFM + E) and control groups. In addition, homeostasis of serum concentrations of calcium, magnesium, and phosphorus was observed to increase in the OVX + RFM + E group rather than in the OVX group without intervention when compared with a control group. Furthermore, fasting and exercise, either alone or concurrently with ovariectomy, induced a non-significant elevation in osteocalcin, parathyroid hormone, and vitamin D3, whereas b-ALP and calcitonin increased significantly compared with those in control rats.
The combination of the Ramadan fasting model and moderate intensity exercises among OVX rats manifested advantageous effects in bone biomarkers compared with OVX rats without intervention. This could be recommended as a lifestyle modification that is protective against osteoporosis, especially in the context of depleted estrogen hormone after menopause.
骨质疏松症是一种慢性骨代谢疾病，影响着全球数百万人，尤其是老年人和绝经后妇女。其特征是骨骼变弱，增加骨折风险，并导致显著的发病率和死亡率。本研究的目的是研究在去卵巢（OVX）大鼠的斋月禁食模型（RFM）中，运动和/或禁食对骨保护作用的报道。
实验干预在去卵巢手术后1个月开始，包括每周进行5次15分钟的运动，速度为18-25米/分钟，和/或从日出到日落（上午6:00-下午7:00）禁食约13小时。测量血清骨代谢生物标志物水平，并测定股骨骨灰和消化血清中的矿物质浓度。此外，使用竞争性酶免疫测定技术测量血清骨碱性磷酸酶（b-ALP）、甲状旁腺激素、骨钙素、降钙素和维生素D3浓度。
与联合组（OVX+RFM+E）和对照组相比，OVX大鼠股骨中的钙、镁和磷矿物质浓度显著降低。此外，与对照组相比，OVX+RFM+E组血清钙、镁和磷浓度的稳态增加，而未干预的OVX组则没有。此外，单独或与去卵巢同时进行的禁食和运动导致骨钙素、甲状旁腺激素和维生素D3有不显著的升高，而与对照大鼠相比，b-ALP和降钙素显著增加。
与未干预的OVX大鼠相比，OVX大鼠的斋月禁食模型和中等强度运动相结合对骨生物标志物表现出有利影响。这可以作为一种预防骨质疏松症的生活方式改变来推荐，特别是在绝经后雌激素激素缺乏的情况下。
