Topuz Bengisu, Gokcen Dincer, Aydin Halil Murat
Bioengineering Division, Institute of Science, Hacettepe University, Ankara, Turkey.
Department of Electrical and Electronics Engineering, Faculty of Engineering, Hacettepe University, Ankara, Turkey.
J Biomed Mater Res A. 2025 Jan;113(1):e37820. doi: 10.1002/jbm.a.37820. Epub 2024 Nov 7.
Many patients suffer from peripheral nerve injury, which can impair their quality of life. Restoring nerve tissue is difficult due to the low ability of nerves to regenerate. Nerve conduits are designed to help peripheral nerve regeneration by providing a scaffold that can match the tissue characteristics, facilitate cellular activities, and be easily implanted. In order to provide a nerve conduit having scaffolding properties, conductance cytocompatibility, we have investigated the potential of channeled structures made of poly (glycerol-sebacate) (PGS) elastomer containing carbon nanofibers (CNFs) in the regeneration of nerve tissue. The first step was to synthesize PGS elastomer and tune its properties to match the nerve tissue. Then, a carbon dioxide laser was used to create micro channels on the elastomer surface for guiding nerve cells. The PGS elastomer was blended with carbon nanofiber (CNF), which was functionalized to bond with the elastomer, to form a conductive structure. The constructs were investigated in terms of cell behavior using PC12 and S42 cell lines. A statistically significant increase in cell proliferation was observed in both cell lines. It was found that the cells began to grow along the canal in places. In terms of elasticity, conductance and cell response, these constructs may be a potential candidate for nerve tissue engineering.
许多患者患有周围神经损伤，这会损害他们的生活质量。由于神经再生能力低，恢复神经组织很困难。神经导管旨在通过提供一种能够匹配组织特性、促进细胞活动并易于植入的支架来帮助周围神经再生。为了提供具有支架特性、导电细胞相容性的神经导管，我们研究了由含碳纳米纤维（CNF）的聚（癸二酸甘油酯）（PGS）弹性体制成的通道结构在神经组织再生中的潜力。第一步是合成PGS弹性体并调整其特性以匹配神经组织。然后，使用二氧化碳激光在弹性体表面创建微通道以引导神经细胞。将PGS弹性体与经功能化以与弹性体结合的碳纳米纤维（CNF）混合，形成导电结构。使用PC12和S42细胞系对构建体的细胞行为进行了研究。在两种细胞系中均观察到细胞增殖有统计学意义的增加。发现细胞开始在某些地方沿着通道生长。就弹性、导电性和细胞反应而言，这些构建体可能是神经组织工程的潜在候选者。
