Werner Tilman, Fahrner Matthias, Schilling Oliver
Institut für klinische Pathologie, Universitätsklinikum Freiburg, Breisacher Straße 115a, 79106, Freiburg im Breisgau, Germany.
Pathologie (Heidelb). 2024 Nov;45(Suppl 1):56-62. doi: 10.1007/s00292-024-01390-x. Epub 2024 Nov 7.
Mass spectrometry (MS)-based proteomics is rapidly transforming pathology research and diagnostics by enabling comprehensive studies of protein expression and post-translational modifications (PTMs).
This article discusses recent advancements in MS-based proteomics, focusing on emerging technologies in sample preparation, MS instrumentation, and data analysis. These developments are scrutinized for their applications in clinical cohort studies and molecular pathology diagnostics.
The article reviews innovations in automated sample preparation, chromatography systems, advanced MS technologies, and proteomic data analysis in the context of pathology. Specific applications such as liquid biopsy, spike-in heavy peptide panels, immunopeptidomics, and PTM screening are highlighted alongside opportunities for data integration.
Recent technological improvements have significantly increased the throughput, precision, and scope of proteomic studies, enabling the analysis of large clinical cohorts and small specimens with unprecedented sensitivity. Advanced MS techniques have broadened applications, opening new avenues for discovery and diagnosis of marker proteins and therapeutic targets.
Advancements in MS-based proteomics have created new opportunities in clinical research and diagnostics. By facilitating more comprehensive and integrated analyses of proteomes, these technologies are set to play a pivotal role in the future of personalized medicine and pathology research.
基于质谱（MS）的蛋白质组学通过对蛋白质表达和翻译后修饰（PTM）进行全面研究，正在迅速改变病理学研究和诊断。
本文讨论基于质谱的蛋白质组学的最新进展，重点关注样品制备、质谱仪器和数据分析方面的新兴技术。对这些进展在临床队列研究和分子病理学诊断中的应用进行了审视。
本文回顾了病理学背景下自动样品制备、色谱系统、先进质谱技术和蛋白质组数据分析方面的创新。重点介绍了液体活检、重肽掺入标准品、免疫肽组学和PTM筛选等具体应用以及数据整合的机会。
最近的技术改进显著提高了蛋白质组学研究的通量、精度和范围，能够以前所未有的灵敏度分析大型临床队列和小样本。先进的质谱技术拓宽了应用范围，为发现和诊断标志物蛋白及治疗靶点开辟了新途径。
基于质谱的蛋白质组学进展为临床研究和诊断创造了新机会。通过促进对蛋白质组进行更全面和综合的分析，这些技术将在个性化医学和病理学研究的未来发挥关键作用。
