一种典型的蛋白质复合物控制免疫稳态和多病原体抗性。

A canonical protein complex controls immune homeostasis and multipathogen resistance.

作者信息

Wu Yue, Xu Weiying, Zhao Guoyan, Lei Ziyao, Li Kui, Liu Jiyun, Huang Shijia, Wang Junli, Zhong Xiangbin, Yin Xin, Wang Yuandong, Zhang Haochen, He Yang, Ye Zian, Meng Yonggang, Chang Xiaoyu, Lin Hui, Wang Xin, Gao Yuanyuan, Chai Jijie, Parker Jane E, Deng Yiwen, Zhang Yu, Gao Mingjun, He Zuhua

机构信息

CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China.

University of the Chinese Academy of Sciences, Beijing 100049, China.

出版信息

Science. 2024 Dec 20;386(6728):1405-1412. doi: 10.1126/science.adr2138. Epub 2024 Nov 7.

DOI:10.1126/science.adr2138

PMID:39509474

Abstract

The calcium (Ca) sensor ROD1 (RESISTANCE OF RICE TO DISEASES1) is a master regulator of immunity in rice. By screening suppressors of mutants, we show that ROD1 governs immune homeostasis by surveilling the activation of a canonical immune pathway. Mutations in (), (), (), and () all abolish enhanced disease resistance of plants. OsTIR catalyzes the production of second messengers 2'-(5″-phosphoribosyl)-5'-adenosine monophosphate (pRib-AMP) and diphosphate (pRib-ADP), which trigger formation of an OsEDS1-OsPAD4-OsADR1 (EPA) immune complex. ROD1 interacts with OsTIR and inhibits its enzymatic activity, whereas mutation of leads to constitutive activation of the EPA complex. Thus, we unveil an immune network that fine-tunes immune homeostasis and multipathogen resistance in rice.

摘要

钙（Ca）传感器ROD1（水稻抗病性1）是水稻免疫的主要调节因子。通过筛选突变体的抑制子，我们发现ROD1通过监测经典免疫途径的激活来控制免疫稳态。在（）、（）、（）和（）中的突变均消除了植物增强的抗病性。OsTIR催化第二信使2'-（5''-磷酸核糖基）-5'-腺苷单磷酸（pRib-AMP）和二磷酸（pRib-ADP）的产生，它们触发OsEDS1-OsPAD4-OsADR1（EPA）免疫复合物的形成。ROD1与OsTIR相互作用并抑制其酶活性，而的突变导致EPA复合物的组成型激活。因此，我们揭示了一个微调水稻免疫稳态和多病原体抗性的免疫网络。