Li Na, Tang Jing, Wang Congxiao, Wang Minghui, Chen Guodong, Jiao Lei, Yang Qinglai, Tan Xiaofeng
Department of Hepatopancreatobiliary Surgery, The First Affiliated Hospital & Center for Molecular Imaging Probe & Hunan Engineering Research Center for Early Diagnosis and Treatment of Liver Cancer, Cancer Research Institute, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China.
Department of Public Health Laboratory Sciences & National Health Commission Key Laboratory of Birth Defect Research and Prevention, Hunan Provincial Maternal and Child Health Care Hospital, Changsha, Hunan 410008, China.
Colloids Surf B Biointerfaces. 2025 Jan;245:114363. doi: 10.1016/j.colsurfb.2024.114363. Epub 2024 Nov 5.
The rational design of nanozymes with highly efficient reactive oxygen species (ROS) generation to overcome the resistant infection microenvironment still faces a significant challenge. Herein, the highly active Fe single-atom nanozymes (Fe SAzymes) with a hierarchically porous nanostructure were prepared through a colloidal silica-induced template method. The proposed Fe SAzymes with satisfactory oxidase (OD)-like and peroxidase (POD)-like activity can transform O and HO to superoxide anion free radical (•O) and hydroxyl radical (•OH), which possess an excellent bactericidal effect. Also, the glutathione peroxidase (GPX)-like activity of Fe SAzymes can consume glutathione in the infection microenvironment, thus facilitating ROS generation to enhance the sterilization effect. Besides, the intrinsic photothermal effect of Fe SAzymes further significantly boosts the enzyme-like activity to generate much more reactive oxygen species for efficient antibacterial therapy. Accordingly, both in vitro and in vivo results indicate that the Fe SAzymes with synergistically photothermal-catalytic performances exhibit satisfactory antibacterial effects and biocompatibility. This work provides new insights into designing highly efficient SAzymes for effective sterilization applications by an amount of ROS generation.
合理设计具有高效活性氧（ROS）生成能力的纳米酶以克服耐药感染微环境仍然面临重大挑战。在此，通过胶体二氧化硅诱导模板法制备了具有分级多孔纳米结构的高活性铁单原子纳米酶（Fe SAzymes）。所制备的具有令人满意的类氧化酶（OD）和类过氧化物酶（POD）活性的Fe SAzymes可将O₂和H₂O₂转化为超氧阴离子自由基（•O₂⁻）和羟基自由基（•OH），具有优异的杀菌效果。此外，Fe SAzymes的类谷胱甘肽过氧化物酶（GPX）活性可消耗感染微环境中的谷胱甘肽，从而促进ROS生成以增强杀菌效果。此外，Fe SAzymes的固有光热效应进一步显著提高类酶活性，以产生更多的活性氧用于高效抗菌治疗。因此，体外和体内结果均表明，具有协同光热催化性能的Fe SAzymes表现出令人满意的抗菌效果和生物相容性。这项工作为通过大量生成ROS设计用于有效杀菌应用的高效单原子纳米酶提供了新的见解。
