Shan Yingli, Cao Feng, Zhao Xin, Luo Jinlong, Mei Haoliang, Zhang Limou, Huang Ying, Yang Yutong, Yan Liangruijie, Huang Yayong, Han Yong, Guo Baolin
State Key Laboratory for Mechanical Behavior of Materials, and Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, China.
State Key Laboratory for Mechanical Behavior of Materials, and Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, China; State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical University, Xi'an, 710032, China.
Biomaterials. 2025 Apr;315:122936. doi: 10.1016/j.biomaterials.2024.122936. Epub 2024 Oct 30.
Procoagulant, antibacterial and analgesic hemostatic hydrogel dressing with high wet tissue adhesion, ultra-high burst pressure, and easy preparation shows huge promising for rapid hemostasis in emergencies, yet it remains a challenge. Herein, we propose hemostatic microsheets based on quaternized chitosan-g-gallic acid (QCS-GA) and oxidized hyaluronic acid (OHA), which merge the benefits of sponges, hydrogels, and powders for rapid hemostasis and efficient wound healing. Specifically, they exhibit a large specific surface area and excellent hydrophilicity, rapidly absorbing blood and self-gelling through electrostatic interaction and Schiff base crosslinking. And this results in dense, porous hydrogel adhesives with superior mechanical properties, adhesion strength, and ultra-high burst pressure. Furthermore, the microsheets are biocompatible, biodegradable, and possess procoagulant, antibacterial, and antioxidant properties. In mouse and rat liver hemorrhage models, the optimized formulation (QCS-GA + OHA4) demonstrated superior hemostatic effects compared to Celox. In particular, QCS-GA + OHA4 microsheets could stop bleeding quickly from rat femoral artery transection and deliver lidocaine to provide analgesia during emergency treatment. Additionally, they promoted wound healing in mouse full-thickness skin defect wound. These easy-to-manufacture hemostatic microsheets are adaptable to irregular wounds, providing a novel solution for rapid hemostasis and wound healing.
具有高湿组织粘附性、超高破裂压力且易于制备的促凝血、抗菌和止痛止血水凝胶敷料在紧急情况下快速止血方面显示出巨大潜力，但仍面临挑战。在此，我们提出了基于季铵化壳聚糖 - 接枝没食子酸（QCS - GA）和氧化透明质酸（OHA）的止血微片，其融合了海绵、水凝胶和粉末的优点以实现快速止血和高效伤口愈合。具体而言，它们具有大的比表面积和优异的亲水性，能快速吸收血液并通过静电相互作用和席夫碱交联实现自凝胶化。这导致形成具有优异机械性能、粘附强度和超高破裂压力的致密多孔水凝胶粘合剂。此外，这些微片具有生物相容性、可生物降解性，并具有促凝血、抗菌和抗氧化性能。在小鼠和大鼠肝出血模型中，优化配方（QCS - GA + OHA4）与Celox相比显示出更优异的止血效果。特别是，QCS - GA + OHA4微片能够迅速止住大鼠股动脉横断出血，并在紧急治疗期间输送利多卡因以提供止痛效果。此外，它们促进了小鼠全层皮肤缺损伤口的愈合。这些易于制造的止血微片适用于不规则伤口，为快速止血和伤口愈合提供了一种新的解决方案。
