Sun Dandan, Liu Yongtong, Li Xiaoqin, Ge Mengqi, Zhu Meiyi, He Yuqin, Hou Zhuocheng
College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Poult Sci. 2024 Dec;103(12):104437. doi: 10.1016/j.psj.2024.104437. Epub 2024 Oct 18.
To deepen our understanding of long-chain fatty acid carbon chain elongation and desaturation in ducks, this study systematically analyzed the transcriptional activities of key gene promoters, including ELOVLs, FADSs, and SCDs. Predictive modeling coupled with statistical analysis revealed a prevalence of binding motifs for transcription factors, particularly those associated with Sp1, NF-1, and C/EBPalpha. Moreover, variation analysis of resequencing data from both wild and domestic ducks, specifically mallards and Pekin ducks, informed targeted mutagenesis within the core promoter regions of ELOVL2, ELOVL5, and ELOVL6. Notably, mutations at positions -56 G>C in ELOVL2, -52 T>C in ELOVL5, and -513 T>C in ELOVL6 significantly diminished transcriptional activity. These findings substantially enhance our understanding of the molecular mechanisms regulating the biosynthesis of long-chain fatty acids in ducks and support future genetic selection initiatives aimed at developing Pekin duck breeds with enhanced nutritional value.
为了加深我们对鸭长链脂肪酸碳链延长和去饱和作用的理解，本研究系统分析了关键基因启动子的转录活性，包括ELOVLs、FADSs和SCDs。预测建模与统计分析相结合，揭示了转录因子结合基序的普遍性，特别是那些与Sp1、NF-1和C/EBPalpha相关的基序。此外，对野鸭和家鸭（特别是绿头鸭和北京鸭）重测序数据的变异分析，为ELOVL2、ELOVL5和ELOVL6核心启动子区域内的靶向诱变提供了信息。值得注意的是，ELOVL2中-56 G>C、ELOVL5中-52 T>C和ELOVL6中-513 T>C处的突变显著降低了转录活性。这些发现极大地增强了我们对鸭长链脂肪酸生物合成调控分子机制的理解，并支持未来旨在培育具有更高营养价值的北京鸭品种的遗传选择计划。
