一种使用离心分析仪测定尿中D-葡萄糖醛酸的半自动方法。

A semiautomated procedure for urinary D-glucaric acid using a centrifugal analyzer.

作者信息

MacNeil M L, Steinberg K K, Yeager P R, Smith S J

出版信息

J Anal Toxicol. 1986 Jan-Feb;10(1):15-7. doi: 10.1093/jat/10.1.15.

DOI:10.1093/jat/10.1.15

Abstract

The most commonly used method for quantifying D-glucaric acid, the enzyme inhibition method, is a two-step procedure. In the first step of the present study, urine was boiled at acidic pH to convert glucaric acid to 1,4-glucarolactone, which inhibits beta-glucuronidase activity. In the second step, inhibition of beta-glucuronidase by the prepared urine was measured. This is an indirect measure of the D-glucaric acid concentration. The second step was adapted to a Cobas-Bio centrifugal analyzer. Values on a reference population obtained by the manual method were highly correlated with values obtained by the semiautomated method described here (r = 0.976, p = 0.25), and precision was comparable by the two methods. The throughput increased from 8 to 20 samples per day when the automated method was used.

摘要

用于定量测定D - 葡萄糖二酸的最常用方法，即酶抑制法，是一个两步程序。在本研究的第一步中，尿液在酸性pH条件下煮沸，将葡萄糖二酸转化为1,4 - 葡萄糖醛酸内酯，后者可抑制β - 葡萄糖醛酸酶的活性。在第二步中，测定制备好的尿液对β - 葡萄糖醛酸酶的抑制作用。这是对D - 葡萄糖二酸浓度的间接测量。第二步采用了Cobas - Bio离心分析仪。通过手动方法在参考人群中获得的值与这里描述的半自动方法获得的值高度相关（r = 0.976，p = 0.25），并且两种方法的精密度相当。使用自动化方法时，每天的样本通量从8个增加到了20个。