Steinberg K K, MacNeil M L, Karon J M, Stehr P A, Neese J W, Needham L L
J Toxicol Environ Health. 1985;16(6):743-52. doi: 10.1080/15287398509530785.
An enzyme-inhibition assay was evaluated and modified to quantify D-glucaric acid in a population exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD). The modified assay combined improvements described separately in previous reports, including pH adjustment by standard addition of buffers rather than by titration, an optimum pH of 2.3 for converting D-glucaric acid to 1,4-glucarolactone, and the use of the relation reciprocal of absorbance versus concentration for calculating unknowns. Reference limits for adult males were 0.06-5.90 mmol D-glucaric acid/mol creatinine and for adult females 0.87-6.23 mmol D-glucaric acid/mol creatinine. Children under the age of 15 yr had a reference range of 0-8.34 mmol D-glucaric acid/mol creatinine. Persons on anticonvulsant therapy excreted from 3 to 10 times the upper reference limits of D-glucaric acid. Urinary concentrations of D-glucaric acid in persons identified as being at high risk for exposure to 2,3,7,8-TCDD in Times Beach, Mo., were not significantly different from concentrations in those identified as being at low risk for such exposure.
对一种酶抑制测定法进行了评估和改进，以定量检测暴露于2,3,7,8-四氯二苯并对二恶英（2,3,7,8-TCDD）人群中的D-葡糖二酸。改进后的测定法结合了先前报告中分别描述的改进措施，包括通过标准添加缓冲液而非滴定来调节pH值、将D-葡糖二酸转化为1,4-葡糖内酯的最佳pH值为2.3，以及使用吸光度倒数与浓度的关系来计算未知物。成年男性的参考限值为0.06 - 5.90 mmol D-葡糖二酸/摩尔肌酐，成年女性为0.87 - 6.23 mmol D-葡糖二酸/摩尔肌酐。15岁以下儿童的参考范围为0 - 8.34 mmol D-葡糖二酸/摩尔肌酐。接受抗惊厥治疗的人排出的D-葡糖二酸是参考上限的3至10倍。在密苏里州时代海滩被确定为高暴露风险的人群中，D-葡糖二酸的尿浓度与被确定为低暴露风险的人群中的浓度没有显著差异。
