Glucaric acid excretion. Analysis of the enzymatic assay and findings in smokers and non-smokers.

Glucaric acid (GA) excretion in urine from cigarette smokers and non-smoking control subjects was determined enzymatically. There was no difference between GA 41.0 +/- 3.2 mumol/g creatinine (Cn; mean +/- SEM) in smokers and GA 43.1 +/- 3.4 mumol/g Cn in the controls. Gas chromatographic analysis of the GA-glucarolactone equilibrium was carried out in an attempt to elucidate the striking discrepancies in previous results.