Hossain Md Mukter, Rahman Md Masudur, Rahman Md Mahfujur, Hossain Hemayet, Begum Ruhena, Chowdhury Md Shahidur Rahman, Islam Md Rafiqul, Uddin Md Bashir
Department of Medicine, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet 3100, Bangladesh.
Department of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet 3100, Bangladesh.
Vet Med Int. 2024 Oct 30;2024:3653654. doi: 10.1155/2024/3653654. eCollection 2024.
This study aimed to determine the seroprevalence and molecular detection of () in dairy cattle using ELISA and PCR techniques. A total of 500 samples (250 blood and 250 milk) were collected from various farms in Sylhet, Bangladesh. The seroprevalence was found to be 5.6% in blood samples, with PCR confirming 1.60% and 2.80% positivity in blood and milk samples, respectively. These findings highlight the zoonotic potential and public health significance of in cattle, suggesting a need for integrated One Health surveillance.
本研究旨在使用酶联免疫吸附测定(ELISA)和聚合酶链反应(PCR)技术测定奶牛中()的血清阳性率并进行分子检测。共从孟加拉国锡尔赫特的各个农场采集了500份样本(250份血液和250份牛奶)。血液样本中的血清阳性率为5.6%,PCR检测证实血液和牛奶样本中的阳性率分别为1.60%和2.80%。这些发现突出了牛中()的人畜共患病潜力和公共卫生意义,表明需要开展一体化的“同一健康”监测。