Dinarvand Negar, Azizi Reza, Rastaghi Sedighe, Karimi Farzaneh, Sheikhi Abdolkarim, Pourfarzam Morteza
Hyperlipidemia Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Basic and Laboratory Sciences, Khomein University of Medical Sciences, Khomein, Iran.
Adv Biomed Res. 2024 Sep 23;13:83. doi: 10.4103/abr.abr_419_23. eCollection 2024.
Disturbances in lipid metabolism are one of the hallmarks of cancer cells. Fatty acid synthesis and oxidation play a crucial role in the proliferation, growth, and survival of cancer cells. Several enzymes are involved in lipid metabolism. MYC is an oncogene and plays various regulatory roles in lipid metabolism. This study aimed to evaluate expression and its association with the expressions of Lipin-1, ACSL4 (enzymes involved in lipid metabolism), in pairs of breast cancer (BC) and adjacent normal tissues to further understand the influence on metabolic regulation.
Fifty-five pairs of samples of BC and noncancerous adjacent tissues were utilized in the present study to analyze , and by quantitative real-time polymerase chain reaction. Further, the expression of Lipin-1 and ACSL4 proteins and a number of other clinicopathologically relevant variables were studied employing immunohistochemistry staining.
expression was substantially higher in BC tissues than in adjacent normal tissues, according to our findings. This upregulation was positively correlated with tumor size and stage. Although expression was not correlated with that of ACSL4 and Lipin-1 expression, this may result from the complex metabolic changes that occur when cells become malignant.
Although further research is required to assess MYC's impact on tumor metabolic regulation, the correlations seen here between , the pathological stage, and tumor size may indicate its prognostic significance in BC. Hence, it may be considered as a potential therapeutic target for further studies.
脂质代谢紊乱是癌细胞的特征之一。脂肪酸合成和氧化在癌细胞的增殖、生长和存活中起着关键作用。几种酶参与脂质代谢。MYC是一种癌基因，在脂质代谢中发挥多种调节作用。本研究旨在评估乳腺癌（BC）及其癌旁正常组织中MYC的表达及其与参与脂质代谢的酶Lipin-1、ACSL4表达的相关性，以进一步了解其对代谢调节的影响。
本研究使用了55对BC组织和癌旁非癌组织样本，通过定量实时聚合酶链反应分析MYC、Lipin-1和ACSL4的表达。此外，采用免疫组织化学染色研究Lipin-1和ACSL4蛋白的表达以及其他一些临床病理相关变量。
根据我们的研究结果，MYC在BC组织中的表达明显高于癌旁正常组织。这种上调与肿瘤大小和分期呈正相关。虽然MYC的表达与ACSL4和Lipin-1的表达无关，但这可能是由于细胞恶变时发生的复杂代谢变化所致。
虽然需要进一步研究来评估MYC对肿瘤代谢调节的影响，但此处观察到的MYC表达、病理分期和肿瘤大小之间的相关性可能表明其在BC中的预后意义。因此，它可被视为进一步研究的潜在治疗靶点。
