Dansoa Constancia S, Anaba Nicholas Y, Zangine Richard T, Kodji Christine, Bonsu Frank A, Abbiw Gideon O, Acheampong Isaac, Wireko Solomon, Domfeh Seth A
Department of Biochemistry and Biotechnology, Faculty of Biosciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Clinical Analysis Laboratory, Department of Biochemistry and Biotechnology, Faculty of Biosciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Int J Microbiol. 2024 Oct 30;2024:1120083. doi: 10.1155/2024/1120083. eCollection 2024.
Type 2 diabetes mellitus (T2DM) patients are at increased risk of infections, such as malignant otitis externa and rhinocerebral mucormycosis, with the urinary tract being the most affected (for example, emphysematous pyelonephritis commonly caused by ). Hence, this study assessed the prevalence of bacteriuria and antibiogram patterns of bacteria isolates among T2DM patients visiting the Ejisu Government Hospital in the Ashanti Region, Ghana. In this cross-sectional study, 58 patients visiting the hospital for routine healthcare were conveniently recruited after obtaining informed consent. Data on sociodemographic characteristics and medical history were obtained using pretested structured questionnaires. Mid-stream urine was collected for bacteria isolation and identification using standard bacterial culture and biochemical tests. Bacteria cultures ≥ 10 CFU/mL were considered significant bacteriuria. The antibiotic sensitivity patterns of the bacteria isolates were evaluated using the Kirby-Bauer disc diffusion method. Bacteriuria was recorded among 15.5% (9/58) of the patients, mainly those with no previous history (77.8%) and no symptoms (55.6%) of urinary tract infections (UTIs). (55.6%) and spp. (44.6%) were primarily isolated from the T2DM patients. All the bacteria isolates ( and spp.) demonstrated the highest resistance to co-trimoxazole and tetracycline (55.6%) and a complete susceptibility to amikacin and levofloxacin (100%). However, 60% of the isolates and 25% of the isolates were multidrug resistant (MDR; resistant to at least one antibiotic agent in three or more antimicrobial categories). The study shows that T2DM patients have bacteria in their urine which are resistant to most common antibiotics, even among those with no history of UTIs; hence, routine bacterial culture and antibiotic sensitivity testing among T2DM patients is recommended for better patient management to reduce the co-morbidities of UTIs.
2型糖尿病（T2DM）患者感染风险增加，如恶性外耳道炎和鼻脑毛霉菌病，其中泌尿系统受影响最大（例如，常见由[此处原文缺失病原体]引起的气肿性肾盂肾炎）。因此，本研究评估了加纳阿散蒂地区埃久苏政府医院就诊的T2DM患者中菌尿症的患病率以及分离出的细菌的抗菌谱模式。在这项横断面研究中，在获得知情同意后，方便地招募了58名到医院进行常规医疗保健的患者。使用预先测试的结构化问卷获取社会人口学特征和病史数据。采集中段尿，通过标准细菌培养和生化试验进行细菌分离和鉴定。细菌培养≥10 CFU/mL被认为是显著菌尿症。使用 Kirby-Bauer 纸片扩散法评估分离出的细菌的抗生素敏感性模式。15.5%（9/58）的患者记录有菌尿症，主要是那些既往无病史（77.8%）且无尿路感染（UTI）症状（55.6%）的患者。[此处原文缺失具体细菌名称]（55.6%）和[此处原文缺失具体细菌名称]属（44.6%）主要从T2DM患者中分离出来。所有分离出的细菌（[此处原文缺失具体细菌名称]和[此处原文缺失具体细菌名称]属）对复方新诺明和四环素的耐药性最高（55.6%），对阿米卡星和左氧氟沙星完全敏感（100%）。然而，60%的[此处原文缺失具体细菌名称]分离株和25%的[此处原文缺失具体细菌名称]属分离株是多重耐药（MDR；对三种或更多抗菌类别中的至少一种抗生素耐药）。该研究表明，T2DM患者尿液中有对大多数常用抗生素耐药的细菌，即使在那些无UTI病史的患者中也是如此；因此，建议对T2DM患者进行常规细菌培养和抗生素敏感性检测，以更好地管理患者，降低UTI的合并症。
