Lv Kaiwen, Liu Naixu, Niu Yani, Song Xiehai, Liu Yongqi, Yue Zhiliang, Ali Muhammad, Guo Qiuyue, Lv Chunyu, Lu Dongdong, Zhang Shaoman, Zhou Yangyan, Li Bosheng
Shandong Provincial Key Laboratory of Precision Molecular Crop Design and Breeding, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in Weifang, Shandong 261325, China.
Salver Academy of Botany, RiZhao, Shandong 262300, China.
Hortic Res. 2024 Aug 20;11(11):uhae237. doi: 10.1093/hr/uhae237. eCollection 2024 Nov.
Propagation through cuttings is a well-established and effective technique for plant multiplication. This study explores the regeneration of poplar roots using spatial transcriptomics to map a detailed developmental trajectory. Mapping of the time-series transcriptome data revealed notable alterations in gene expression during root development, particularly in the activation of cytokinin-responsive genes. Our analysis identified six distinct clusters during the second and third stages, each corresponding to specific anatomical regions with unique gene expression profiles. Auxin response -elements (AuxREs) were prevalent in the promoters of these cytokinin-responsive genes, indicating a regulatory interplay between auxin and cytokinin. Pseudo-temporal trajectory analysis mapped the differentiation from cambium cells to root primordium cells, revealing a complex pattern of cell differentiation. and emerged as potential novel biomarkers for enhancing root regeneration, with distinct spatial expression patterns confirmed by hybridization. This comprehensive spatial analysis enhances our understanding of the molecular interactions driving root regeneration and provides insights for improving plant propagation techniques.
通过扦插繁殖是一种成熟且有效的植物繁殖技术。本研究利用空间转录组学探索杨树根系的再生，以绘制详细的发育轨迹。对时间序列转录组数据的映射揭示了根系发育过程中基因表达的显著变化，特别是细胞分裂素响应基因的激活。我们的分析在第二和第三阶段识别出六个不同的簇，每个簇对应具有独特基因表达谱的特定解剖区域。生长素响应元件（AuxREs）在这些细胞分裂素响应基因的启动子中普遍存在，表明生长素和细胞分裂素之间存在调控相互作用。伪时间轨迹分析绘制了形成层细胞到根原基细胞的分化过程，揭示了复杂的细胞分化模式。[此处可能有两个未给出的内容]作为增强根系再生的潜在新型生物标志物出现，通过[此处可能有未给出的杂交技术]杂交证实了其独特的空间表达模式。这种全面的空间分析增强了我们对驱动根系再生的分子相互作用的理解，并为改进植物繁殖技术提供了见解。
