四核糖核苷酸rCpGpCpG形成左手Z-RNA双螺旋。
The tetraribonucleotide rCpGpCpG forms a left-handed Z-RNA double-helix.
作者信息
Davis P W, Hall K, Cruz P, Tinoco I, Neilson T
出版信息
Nucleic Acids Res. 1986 Feb 11;14(3):1279-91. doi: 10.1093/nar/14.3.1279.
Abstract
NMR and circular dichroism studies show that the RNA tetranucleotide rCpGpCpG can form a Z-RNA left-handed double-helix. In 1.0 M NaClO4, circular dichroism measurements indicate that the tetramer is in the A-form. In 6.0 M NaClO4, there is a characteristic change in the circular dichroism, indicating that the tetramer adopts a left-handed Z-form. This conformation is verified by phosphorus and proton NMR studies. The 31P spectrum shows a large downfield shift in one of the resonances upon an increase in salt concentration. Proton nuclear Overhauser effect (NOE) experiments indicate that the guanosines are in the syn conformation. These results are consistent with the formation of a Z-form double-helix.
摘要
核磁共振（NMR）和圆二色性研究表明，RNA四核苷酸rCpGpCpG可形成Z-RNA左手双螺旋结构。在1.0 M高氯酸钠（NaClO4）中，圆二色性测量表明该四聚体呈A构象。在6.0 M高氯酸钠中，圆二色性出现特征性变化，表明该四聚体采用左手Z构象。这种构象通过磷和质子核磁共振研究得到验证。31P谱显示，随着盐浓度增加，其中一个共振峰出现大幅向低场移动。质子核Overhauser效应（NOE）实验表明，鸟苷处于顺式构象。这些结果与Z型双螺旋结构的形成一致。
相似文献
1
The tetraribonucleotide rCpGpCpG forms a left-handed Z-RNA double-helix.
Nucleic Acids Res. 1986 Feb 11;14(3):1279-91. doi: 10.1093/nar/14.3.1279.
2
High salt solution structure of a left-handed RNA double helix.
Nucleic Acids Res. 2004 Aug 3;32(13):4044-54. doi: 10.1093/nar/gkh736. Print 2004.
3
Z-RNA: the solution NMR structure of r(CGCGCG).
Biopolymers. 1990 Jan;29(1):109-22. doi: 10.1002/bip.360290116.
4
Conformational analysis of r(CGCGCG) in aqueous solution: an A-type double helical conformation studied by two-dimensional nuclear Overhauser effect spectroscopy.
Nucleic Acids Res. 1984 May 25;12(10):4323-38. doi: 10.1093/nar/12.10.4323.
5
'Z-RNA'--a left-handed RNA double helix.
Nature. 1984;311(5986):584-6. doi: 10.1038/311584a0.
6
Spectroscopic studies of (m5dC-dG)3: thermal stability of B- and Z-forms.
Nucleic Acids Res. 1983 Jul 11;11(13):4453-66. doi: 10.1093/nar/11.13.4453.
7
NMR and CD studies on an oligonucleotide containing N4-methylcytosine.
Nucleic Acids Res. 1987 Mar 11;15(5):2191-201. doi: 10.1093/nar/15.5.2191.
8
L-nucleotides and 8-methylguanine of d(C1m8G2C3G4C5LG6LC7G8C9G10)2 act cooperatively to promote a left-handed helix under physiological salt conditions.
Nucleic Acids Res. 2003 Dec 1;31(23):6986-95. doi: 10.1093/nar/gkg893.
9
Evidence for Z-form RNA by vacuum UV circular dichroism.
Nucleic Acids Res. 1985 Jul 11;13(13):4983-9. doi: 10.1093/nar/13.13.4983.
10
Conformation of ribooligonucleotide duplexes containing an alternating C-G sequence which show an unusual circular dichroism spectrum.
J Biol Chem. 1984 Feb 10;259(3):1390-3.
引用本文的文献
1
Zα Domain of ADAR1 Binds to an A-Form-like Nucleic Acid Duplex with Low Micromolar Affinity.
Biochemistry. 2024 Mar 19;63(6):777-787. doi: 10.1021/acs.biochem.3c00636. Epub 2024 Mar 4.
2
Z-Form Adoption of Nucleic Acid is a Multi-Step Process Which Proceeds through a Melted Intermediate.
J Am Chem Soc. 2024 Jan 10;146(1):677-694. doi: 10.1021/jacs.3c10406. Epub 2023 Dec 22.
3
Deconvolution of conformational exchange from Raman spectra of aqueous RNA nucleosides.
Commun Chem. 2020 May 6;3(1):56. doi: 10.1038/s42004-020-0298-x.
4
Z-RNA biology: a central role in the innate immune response?
RNA. 2023 Mar;29(3):273-281. doi: 10.1261/rna.079429.122. Epub 2023 Jan 3.
5
Z-nucleic acids: Uncovering the functions from past to present.
Eur J Immunol. 2022 Nov;52(11):1700-1711. doi: 10.1002/eji.202249968. Epub 2022 Oct 10.
6
Adenosine-to-inosine editing of endogenous Z-form RNA by the deaminase ADAR1 prevents spontaneous MAVS-dependent type I interferon responses.
Immunity. 2021 Sep 14;54(9):1961-1975.e5. doi: 10.1016/j.immuni.2021.08.011.
7
Conformational adaptation of UNCG loops upon crowding.
RNA. 2019 Nov;25(11):1522-1531. doi: 10.1261/rna.072694.119. Epub 2019 Aug 19.
8
Comparison of the solution and crystal conformations of (G + C)-rich fragments of DNA.
Biophys J. 1996 Sep;71(3):1530-8. doi: 10.1016/S0006-3495(96)79355-4.
本文引用的文献
1
High-salt d(CpGpCpG), a left-handed Z' DNA double helix.
Nature. 1980 Aug 7;286(5773):567-73. doi: 10.1038/286567a0.
2
Right-handed and left-handed DNA: studies of B- and Z-DNA by using proton nuclear Overhauser effect and P NMR.
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1413-7. doi: 10.1073/pnas.79.5.1413.
3
Conformational analysis of r(CGCGCG) in aqueous solution: an A-type double helical conformation studied by two-dimensional nuclear Overhauser effect spectroscopy.
Nucleic Acids Res. 1984 May 25;12(10):4323-38. doi: 10.1093/nar/12.10.4323.
4
Helix geometry and hydration in an A-DNA tetramer: IC-C-G-G.
J Mol Biol. 1984 Apr 25;174(4):663-95. doi: 10.1016/0022-2836(84)90089-5.
5
1H NMR and circular dichroism studies of the B and Z conformations of the self-complementary deoxyhexanucleotide d(m5C-G-C-G-m5-C-G): mechanism of the Z-B-coil transitions.
Biochemistry. 1984 Mar 27;23(7):1362-71. doi: 10.1021/bi00302a005.
6
Conformation of ribooligonucleotide duplexes containing an alternating C-G sequence which show an unusual circular dichroism spectrum.
J Biol Chem. 1984 Feb 10;259(3):1390-3.
7
'Z-RNA'--a left-handed RNA double helix.
Nature. 1984;311(5986):584-6. doi: 10.1038/311584a0.
8
Sequence dependence of conformations of self-complementary duplex tetradeoxynucleotides containing cytosine and guanine.
Biochemistry. 1984 Jul 3;23(14):3202-7. doi: 10.1021/bi00309a014.
9
Relative stability of guanosine-cytidine diribonucleotide cores: a 1H NMR assessment.
Biochemistry. 1984 Jun 5;23(12):2656-62. doi: 10.1021/bi00307a018.
10
B,Z conformations and mechanism of the Z-B-coil transitions of the self-complementary deoxy-hexanucleotide d(C-G-m5C-G-C-G) by 1H-NMR and CD spectroscopy.
J Biomol Struct Dyn. 1984 Jun;1(6):1347-71. doi: 10.1080/07391102.1984.10507525.
Zotero 插件