Wang Xinmin, Huang Lizhen, Du Qing, Li Jing, Zheng Qin, Chen Yingchong, Yue Pengfei
Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Chinese Medicine, Nanchang 330004, China.
Jiangxi Provincial Institute of Traditional Chinese Medicine, Nanchang 330007, China.
Int J Pharm. 2024 Dec 25;667(Pt B):124918. doi: 10.1016/j.ijpharm.2024.124918. Epub 2024 Nov 7.
To improve the retention time and skin-whitening efficacy of Atractylodes macrocephale essential oil (AMO), a novel Pickering emulsion based nanogel loaded with AMO (AMO-PEG) was successfully developed. This formulation employed nano-pearl powder (NPP) as the particle stabilizer for the Pickering emulsion and Bletilla striata polysaccharide (BSP) as the gel matrix. The pH, rheological properties, hardness, and elasticity of AMO-PEG were affected by the ratio of AMO-Pickering emulsion (AMO-PE) to BSP gel matrix. The results showed that AMO-PEG exhibited solid-like behavior and was capable of forming nanogels when the ratio of AMO-PE to BSP was 1:1. AMO-PE and AMO-PEG are two different dosage forms in the preparation of AMO. The effects of varying dosage forms on AMO were evaluated by in vitro transdermal release, skin irritation test, and skin-whitening effect. AMO-PEG conforms to the zero-order kinetic equation (R = 0.9189). The skin retention rate of AMO-PEG was 1.37 times higher than that of AMO-PE, indicating that AMO-PEG could continuously and slowly exert the whitening effect of the drugs. Compared with AMO-PE, AMO-PEG significantly increased the inhibition of tyrosinase activity and melanogenesis in B16F10 cells. AMO-PEG can promote the inhibition of B16F10 cells and improve the whitening effect of AMO and BSP. In conclusion, the Pickering emulsion based nanogel appears to be a promising strategy for enhancing the skin-whitening efficacy of both AMO and BSP.
为提高白术精油（AMO）的滞留时间和美白功效，一种负载AMO的新型Pickering乳液基纳米凝胶（AMO-PEG）被成功研制出来。该制剂采用纳米珍珠粉（NPP）作为Pickering乳液的颗粒稳定剂，白及多糖（BSP）作为凝胶基质。AMO-PEG的pH值、流变学性质、硬度和弹性受AMO-Pickering乳液（AMO-PE）与BSP凝胶基质比例的影响。结果表明，当AMO-PE与BSP的比例为1:1时，AMO-PEG呈现类固体行为并能够形成纳米凝胶。AMO-PE和AMO-PEG是AMO制备中的两种不同剂型。通过体外透皮释放、皮肤刺激性试验和美白效果评估了不同剂型对AMO的影响。AMO-PEG符合零级动力学方程（R = 0.9189）。AMO-PEG的皮肤滞留率比AMO-PE高1.37倍，表明AMO-PEG能够持续缓慢地发挥药物的美白作用。与AMO-PE相比，AMO-PEG显著增强了对B16F10细胞酪氨酸酶活性和黑色素生成的抑制作用。AMO-PEG能够促进对B16F10细胞的抑制作用，提高AMO和BSP的美白效果。总之，Pickering乳液基纳米凝胶似乎是增强AMO和BSP美白功效的一种有前景的策略。
