College of Food Science, Sichuan Agricultural University, Ya'an 625014, China.
College of Food Science, Sichuan Agricultural University, Ya'an 625014, China.
Bioresour Technol. 2025 Jan;416:131774. doi: 10.1016/j.biortech.2024.131774. Epub 2024 Nov 7.
This study investigated the conversion of okara insoluble dietary fiber (IDF) to soluble dietary fiber (SDF) using high-pressure homogenization (HPH) combined with microbial fermentation. The conversion mechanism was analyzed from four aspects: correlation, proteomics, component and structure under. Results indicated a negative correlation between pH and SDF yield (r = -0.9885, p < 0.05), while cellulase and xylanase showed a positive correlation with SDF yield (p < 0.05). Proteomic analysis identified 22 key enzymes involved in IDF degradation. According to the composition and structure, the combined treatment effectively reduced the aggregation of IDF, promoting its transformation into SDF. HPH treatment primarily acted on the hemicellulose fractions in the amorphous region, while microbial fermentation broke hydrogen bonds between hydroxyl groups in the crystalline regions of cellulose, enhancing the conversion of more exposed cellulose. This study provided theoretical support for the development and utilization of okara IDF.
本研究采用高压均质（HPH）联合微生物发酵的方法将豆渣不溶性膳食纤维（IDF）转化为可溶性膳食纤维（SDF）。从相关性、蛋白质组学、成分和结构四个方面分析了转化机制。结果表明，pH 值与 SDF 得率呈负相关（r = -0.9885，p < 0.05），而纤维素酶和木聚糖酶与 SDF 得率呈正相关（p < 0.05）。蛋白质组学分析鉴定了 22 种参与 IDF 降解的关键酶。根据组成和结构，联合处理有效地减少了 IDF 的聚集，促进了其向 SDF 的转化。HPH 处理主要作用于无定形区的半纤维素部分，而微生物发酵打破了纤维素结晶区中羟基之间的氢键，增强了更多暴露的纤维素的转化。本研究为豆渣 IDF 的开发利用提供了理论支持。
