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制备并表征钙掺杂氧化石墨烯-壳聚糖纳米载体以增强 MCF-7 细胞系中的基因传递。

Preparation and characterization of calcium-doped graphene oxide-chitosan Nanocarrier to enhance the gene delivery in MCF-7 cell line.

机构信息

Department of Pharmaceutical Biotechnology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran.

Dental Implant Research Center, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran.

出版信息

Sci Rep. 2024 Nov 10;14(1):27434. doi: 10.1038/s41598-024-78958-3.

DOI:10.1038/s41598-024-78958-3
PMID:39521829
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11550409/
Abstract

Gene delivery has emerged as a novel and effective method in the treatment of malignancies within medical interventions by applying nanotechnology. Consequently, the development of appropriate nanocarriers is a key focus of this research. Dynamic light scattering (DLS), fourier transform infrared (FT-IR) spectroscopy, x-ray diffraction (XRD), and thermal gravimetric analysis (TGA) were employed for the characterization of the synthesized nanocarrier. Furthermore, to assess the gene transfer capability of the nanocarrier, various techniques such as gel retardation assay, nuclease resistance assay, cytotoxicity assay, flow cytometry, and transfection were employed. The average particle size and zeta potential of the GO-CS@Ca nanocarrier were obtained as 319.8 nm and + 92.8 mv, respectively. In the gel retardation test, it was observed that pDNA was effectively condensed by the GO-CS@Ca nanocarrier. The results of the MTT assay indicated that both GO-CS@Ca nanocarrier and the GO-CS@Ca/pDNA nanoplex with low toxicity. In flow cytometry analysis, it was observed that the complexation of pDNA with the GO-CS@Ca nanocarrier resulted in effective gene delivery to the MCF-7 cell line and consequently increased apoptosis induction.

摘要

基因传递在医学干预中已成为治疗恶性肿瘤的一种新颖而有效的方法，通过应用纳米技术。因此，开发合适的纳米载体是这项研究的重点。动态光散射（DLS）、傅里叶变换红外（FT-IR）光谱、X 射线衍射（XRD）和热重分析（TGA）用于合成纳米载体的表征。此外，为了评估纳米载体的基因转移能力，采用了凝胶阻滞实验、核酸酶抗性实验、细胞毒性实验、流式细胞术和转染等多种技术。GO-CS@Ca 纳米载体的平均粒径和 Zeta 电位分别为 319.8nm 和+92.8mv。在凝胶阻滞实验中，观察到 GO-CS@Ca 纳米载体有效地浓缩了 pDNA。MTT 试验的结果表明，GO-CS@Ca 纳米载体和具有低毒性的 GO-CS@Ca/pDNA 纳米复合物。在流式细胞术分析中，观察到 pDNA 与 GO-CS@Ca 纳米载体的复合物有效地递送到 MCF-7 细胞系，从而诱导细胞凋亡增加。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/11550409/c0c5e35356ec/41598_2024_78958_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/11550409/d10f7b3b3f96/41598_2024_78958_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/11550409/dfb56f1166fa/41598_2024_78958_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/11550409/82b39ad92e81/41598_2024_78958_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/11550409/bb381a46799b/41598_2024_78958_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/11550409/a76a6fdba4c1/41598_2024_78958_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/11550409/759f98e5d9e9/41598_2024_78958_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/11550409/0a0387c8b76b/41598_2024_78958_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/11550409/c0c5e35356ec/41598_2024_78958_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/11550409/d10f7b3b3f96/41598_2024_78958_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/11550409/dfb56f1166fa/41598_2024_78958_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/11550409/82b39ad92e81/41598_2024_78958_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/11550409/bb381a46799b/41598_2024_78958_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/11550409/a76a6fdba4c1/41598_2024_78958_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/11550409/759f98e5d9e9/41598_2024_78958_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/11550409/0a0387c8b76b/41598_2024_78958_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc8a/11550409/c0c5e35356ec/41598_2024_78958_Fig8_HTML.jpg

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