Song Z, Han L, Lin Z, Shi G
School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Zhongshan 528400, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2024 Oct 20;44(10):1887-1897. doi: 10.12122/j.issn.1673-4254.2024.10.07.
To observe the effects of restricted and high-fat diets on behavioral changes of wild-type () and transgenic mice carrying -A187V mutation () with short sleep durations.
and C57BL/6 mice were randomized into normal chow group (25 and 26 mice for behavioral monitoring), odor retention fasting group (17 and 19 mice for behavioral monitoring; 6 mice and 6 mice for EEG/EMG monitoring), absolute fasting group (6 and 4-5 mice for behavioral monitoring; 6 and 6 mice for EEG/EMG monitoring), and high-fat diet group (6 and 7 mice for behavioral monitoring; 6 and 6 mice for EEG/EMG monitoring). Electrodes for EEG and muscle activity monitoring were implanted on the skulls of the mice. After 24 h of odor retention fasting, absolute fasting, or high-fat feeding, the mice were observed for behavioral changes adapted to diet changes.
In odor retention fasting experiment, mice exhibited more stable fluctuations of activities with mildly reduced movement and prolonged sleep duration, indicating enhanced starvation resistance. In absolute fasting experiment, mice showed significantly increased nighttime water intake, improved rhythmicity in water intake (frequent intakes in small amounts), and increased duration of non-rapid eye movement sleep (NREM). In the high-fat diet experiment, mice showed higher levels of activity with increased instances of nighttime rearing, longer movement distances, and increased rapid eye movement sleep during daytime.
mice can quickly respond to environmental changes and under restricted dietary conditions, they can conserve energy by increasing sleep to maintain energy homeostasis but show higher levels of activity under high-fat dietary conditions.
观察限制饮食和高脂饮食对野生型（）和携带 -A187V 突变（）且睡眠时间短的转基因小鼠行为变化的影响。
将和 C57BL/6 小鼠随机分为正常饮食组（分别有 25 只和 26 只小鼠用于行为监测）、气味保留禁食组（分别有 17 只和 19 只小鼠用于行为监测；分别有 6 只和 6 只小鼠用于脑电图/肌电图监测）、绝对禁食组（分别有 6 只和 4 - 5 只小鼠用于行为监测；分别有 6 只和 6 只小鼠用于脑电图/肌电图监测）和高脂饮食组（分别有 6 只和 7 只小鼠用于行为监测；分别有 6 只和 6 只小鼠用于脑电图/肌电图监测）。将用于脑电图和肌肉活动监测的电极植入小鼠颅骨。在进行气味保留禁食、绝对禁食或高脂喂养 24 小时后，观察小鼠适应饮食变化的行为变化。
在气味保留禁食实验中，小鼠活动波动更稳定，运动略有减少，睡眠时间延长，表明抗饥饿能力增强。在绝对禁食实验中，小鼠夜间饮水量显著增加，饮水节律改善（少量频繁饮水），非快速眼动睡眠（NREM）时间增加。在高脂饮食实验中，小鼠活动水平较高，夜间竖毛次数增加，移动距离更长，白天快速眼动睡眠时间增加。
小鼠能快速响应环境变化，在饮食受限条件下，它们可通过增加睡眠来保存能量以维持能量稳态，但在高脂饮食条件下活动水平较高。
