Cohen Netanel, Rabinowitch Ithai
Department of Medical Neurobiology, Institute for Medical Research Israel-Canada, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 9112002, Israel.
iScience. 2024 Oct 10;27(11):111147. doi: 10.1016/j.isci.2024.111147. eCollection 2024 Nov 15.
Memory consolidation following learning is a dynamic and complex process comprising several transitions between distinct memory phases. Although memory consolidation has been studied extensively, it remains difficult to draw an integral description that can delimit the transition points between specific memory phases at the behavioral, neuronal, and genetic levels. To this end, we have developed a rapid and robust aversive conditioning protocol for the nematode worm , tracing memory consolidation within the first hour post conditioning and then up to 18 h post conditioning. This made it possible to uncover time-dependent involvement of primary sensory neurons, transcription and translation processes, and diverse gene populations in memory consolidation. The change in neuronal valence was strong enough to induce second order conditioning, and was amenable to considerable modulation in specific mutant strains. Together, our work lends memory consolidation to detailed temporal and spatial analysis, advancing system-wide understanding of learning and memory.
学习后的记忆巩固是一个动态且复杂的过程，包括在不同记忆阶段之间的几次转变。尽管对记忆巩固进行了广泛研究，但仍难以得出一个完整的描述，以界定行为、神经元和基因水平上特定记忆阶段之间的转变点。为此，我们为线虫开发了一种快速且可靠的厌恶条件化方案，追踪条件化后第一小时内以及之后长达18小时的记忆巩固过程。这使得揭示初级感觉神经元、转录和翻译过程以及不同基因群体在记忆巩固中的时间依赖性参与成为可能。神经元效价的变化足够强烈，足以诱导二阶条件化，并且在特定突变株中易于受到显著调节。总之，我们的工作为记忆巩固提供了详细的时空分析，推动了对学习和记忆的全系统理解。
