Song Xin-Jie, Ye Fei, Zhang Yao, Sun Juan, Shentu Xuping, Yu Xiaoping, Li Wei, Wu Yuan-Feng
Zhejiang Provincial Key Laboratory of Chemical and Biological Processing Technology for Agricultural Products, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Liuxia Street Number 318, Hangzhou 310023, PR China.
Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Science, China Jiliang University, Hangzhou, China.
Food Chem X. 2024 Oct 21;24:101911. doi: 10.1016/j.fochx.2024.101911. eCollection 2024 Dec 30.
In this study, a fluorescence detection method combining aptamer-modified up-conversion nanoparticles (UCNPs) and magnetic nanoparticles (MNPs) was developed for detection of Clenbuterol (CLB). The aptamer-modified magnetic NPs captured CLB, which reacted with the aptamer-modified UCNPs and generated a sandwich complex. The aptamer-modified UCNPs acted as a fluorescence source. The MNP-CLB-UCNP complex was retrieved from the solution using an magnetic field, and the fluorescence intensity was detected by fluorescence spectrophotometry with excitation and emission spectra at 980 nm and in the 400-800 nm region, respectively. The results showed that the fluorescence intensity gradually increased with increasing concentrations of CLB with a good specify. The method was highly sensitive for the quantification of CLB, with a limit of detection of 0.304 ng mL. The recovery rate of CLB from pork samples ranged from 84 % to 94.87 %. This fluorescence method enables the sensitive, precise, and accurate quantification of CLB residues in pork samples.
在本研究中，开发了一种结合适配体修饰的上转换纳米粒子（UCNPs）和磁性纳米粒子（MNPs）的荧光检测方法用于检测克伦特罗（CLB）。适配体修饰的磁性纳米粒子捕获CLB，其与适配体修饰的UCNPs反应并生成夹心复合物。适配体修饰的UCNPs作为荧光源。使用磁场从溶液中回收MNP-CLB-UCNP复合物，并通过荧光分光光度法检测荧光强度，激发光谱和发射光谱分别在980nm和400-800nm区域。结果表明，荧光强度随CLB浓度的增加而逐渐增加，具有良好的特异性。该方法对CLB的定量高度灵敏，检测限为0.304ng/mL。猪肉样品中CLB的回收率在84%至94.87%之间。这种荧光方法能够灵敏、精确且准确地定量猪肉样品中的CLB残留。
