Liu Yi-Shi, Miao Yu-Long, Dou Yue, Yang Ze-Hui, Sun Wenhao, Zhou Xiaoman, Li Zijie, Hideki Nakanishi, Gao Xiao-Dong, Fujita Morihisa
Key Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Ministry of Education, Jiangnan University, Wuxi, Jiangsu, 214122, China.
Key Laboratory for Southwest Microbial Diversity of the Ministry of Education, School of Life Sciences, Yunnan University, Kunming, Yunnan, 650021, China.
Glycoconj J. 2024 Dec;41(6):361-370. doi: 10.1007/s10719-024-10171-w. Epub 2024 Nov 12.
Glycoconjugates, including glycans on proteins and lipids, have obtained significant attention due to their critical roles in both intracellular and intercellular biological functions and processes. Notably, recent discoveries have revealed the presence of glycosylated RNAs (glycoRNAs) on cell surfaces. Despite the well-characterized roles of RNA modifications, RNA glycosylation remains relatively unexplored. In this study, we investigate the relationship between N-glycosylation and RNA glycosylation. Using a recombinant Siglec11-Fc as a probe, we detected surface sialylated glycoRNAs in human cell lines and identified their dependency on the catalytic isoforms of the oligosaccharyltransferase (OST) complex, implicating STT3A-dependent protein glycosylation as a predominant contributor for affecting indirect generation of glycoRNAs. Additionally, perturbations in N-glycan biosynthesis pathways or changes in N-glycan structure impact surface sialylated glycoRNA levels, indicating a regulatory role of glycan metabolic pathways in RNA glycosylation. Together, our results underscore the intricate relationship between protein N-glycosylation and processing and RNA biology.
糖缀合物，包括蛋白质和脂质上的聚糖，因其在细胞内和细胞间生物学功能及过程中的关键作用而备受关注。值得注意的是，最近的发现揭示了细胞表面存在糖基化RNA（glycoRNAs）。尽管RNA修饰的作用已得到充分表征，但RNA糖基化仍相对未被深入研究。在本研究中，我们调查了N - 糖基化与RNA糖基化之间的关系。使用重组Siglec11 - Fc作为探针，我们在人类细胞系中检测到表面唾液酸化的glycoRNAs，并确定了它们对寡糖基转移酶（OST）复合物催化异构体的依赖性，这表明STT3A依赖性蛋白质糖基化是影响glycoRNAs间接生成的主要因素。此外，N - 聚糖生物合成途径的扰动或N - 聚糖结构的变化会影响表面唾液酸化glycoRNA水平，表明聚糖代谢途径在RNA糖基化中具有调节作用。总之，我们的结果强调了蛋白质N - 糖基化与加工和RNA生物学之间的复杂关系。
