Vine Tyler, Carpenter Rob E, Bridges Debbie
Department of Research, Advanta Genetics, 10935 CR 159, Tyler, TX 75703, United States.
Soules College of Business, The University of Texas at Tyler, 3900 University Boulevard, Tyler, TX 75799, United States.
Data Brief. 2024 Oct 18;57:111036. doi: 10.1016/j.dib.2024.111036. eCollection 2024 Dec.
This dataset provides information from a study that validates a method for non-invasive urine collection utilizing sodium polyacrylate-based diapers, designed for polymerase chain reaction (PCR) detection of uropathogens. The dataset includes samples from 17 participants, comprising seven clinically contrived samples inoculated with known uropathogens and ten samples collected from volunteers wearing sodium polyacrylate-based diapers. The study involved optimizing urine extraction from the diaper matrices, ensuring minimal loss of diagnostic sensitivity for downstream quantitative PCR (qPCR) analysis. The qPCR targeted 22 uropathogens, six fungal species, and 18 antimicrobial resistance (AMR) genes, providing comprehensive molecular characterization of urinary pathogens. Comparative analyses were performed between diaper-derived and standard urine samples, with data demonstrating high concordance in detection outcomes, despite a modest average reduction in qPCR sensitivity (ΔCt of -1.65). Matrix effects were evaluated to determine the impact of the diaper material on qPCR amplification efficiency. The dataset is valuable for further research into non-invasive diagnostic techniques for urinary tract infections (UTIs), particularly for populations unable to provide midstream urine samples, such as infants and elderly individuals. Potential reuse of this dataset includes evaluating non-invasive urine collection efficacy, exploring the interaction between sample matrices and molecular diagnostics, and assessing the scalability of diaper-based sampling methods in diverse clinical settings.
该数据集提供了一项研究的信息，该研究验证了一种利用基于聚丙烯酸钠的尿布进行非侵入性尿液收集的方法，该方法用于通过聚合酶链反应（PCR）检测尿路病原体。该数据集包括17名参与者的样本，其中有7个接种了已知尿路病原体的临床人工样本，以及10个从穿着基于聚丙烯酸钠尿布的志愿者身上采集的样本。该研究涉及优化从尿布基质中提取尿液，确保对下游定量PCR（qPCR）分析的诊断敏感性损失最小。qPCR针对22种尿路病原体、6种真菌和18种抗菌药物耐药性（AMR）基因，提供尿路病原体的全面分子特征。对尿布衍生样本和标准尿液样本进行了比较分析，数据表明检测结果高度一致，尽管qPCR敏感性平均略有降低（ΔCt为-1.65）。评估了基质效应，以确定尿布材料对qPCR扩增效率的影响。该数据集对于进一步研究尿路感染（UTI）的非侵入性诊断技术具有重要价值，特别是对于无法提供中段尿样本的人群，如婴儿和老年人。该数据集的潜在再利用包括评估非侵入性尿液收集的有效性、探索样本基质与分子诊断之间的相互作用，以及评估基于尿布的采样方法在不同临床环境中的可扩展性。
