Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing 100193, China.
School of Food Science and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China.
J Agric Food Chem. 2024 Nov 27;72(47):26165-26177. doi: 10.1021/acs.jafc.4c06524. Epub 2024 Nov 13.
Human milk oligosaccharides could prevent pathogenic bacterial infections in neonates; however, direct in vivo anti-infection evidence was still lacking. Here, we systematically evaluated the effects of 2'-fucosyllactose (2'-FL) and 3'-sialyllactose (3'-SL) on the structural development and functional maturation in neonates and their defense against enteroaggregative infection. It was found that supplementation with 2'-FL and 3'-SL improved the resistance of weaned mice to enteroaggregative . The mechanism related to the promotion of 2'-FL and 3'-SL in the maturation of intestinal mucosal immunity by promoting stem cell differentiation, mucus layer integrity, and tight junction formation. 2'-FL and 3'-SL significantly increased the ratio of Th1 and Treg cells in the lamina propria, contents of short-chain fatty acids, as well as the serum content of IgA. This study lays a theoretical basis for the application of 2'-FL and 3'-SL in infant formula, as well as the development of intestinal health products.
人乳寡糖可以预防新生儿的致病性细菌感染;然而，直接的体内抗感染证据仍然缺乏。在这里，我们系统地评估了 2'-岩藻糖基乳糖(2'-FL)和 3'-唾液酸乳糖(3'-SL)对新生动物结构发育和功能成熟的影响及其对肠聚集性感染的防御作用。结果发现，补充 2'-FL 和 3'-SL 可提高断奶小鼠对肠聚集性的抵抗力。其机制与促进干细胞分化、黏液层完整性和紧密连接形成，从而促进 2'-FL 和 3'-SL 促进肠道黏膜免疫成熟有关。2'-FL 和 3'-SL 显著增加了固有层中 Th1 和 Treg 细胞的比例、短链脂肪酸的含量以及血清 IgA 的含量。本研究为 2'-FL 和 3'-SL 在婴儿配方奶粉中的应用以及肠道健康产品的开发奠定了理论基础。
