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表达肿瘤生长因子-β1（TGFβ1）的杆状病毒纳米穿梭体增强血管创伤愈合的治疗效果：设计与体外分析

Baculovirus Expressing Tumor Growth Factor-β1 (TGFβ1) Nanoshuttle Augments Therapeutic Effects for Vascular Wound Healing: Design and In Vitro Analysis.

作者信息

Islam Paromita, Abosalha Ahmed, Schaly Sabrina, Boyajian Jacqueline L, Santos Madison, Makhlouf Stephanie, Renesteen Editha, Kassab Amal, Shum-Tim Cedrique, Shum-Tim Dominique, Prakash Satya

机构信息

Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine and Health Sciences, McGill University, Montreal, Quebec H3A 2B4, Canada.

Pharmaceutical Technology Department, Faculty of Pharmacy, Tanta University, Tanta Al-Geish St., the Medical Campus, Tanta 31527, Egypt.

出版信息

ACS Pharmacol Transl Sci. 2024 Oct 25;7(11):3419-3428. doi: 10.1021/acsptsci.4c00509. eCollection 2024 Nov 8.

DOI:10.1021/acsptsci.4c00509
PMID:39539270
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11555499/
Abstract

One of the major challenges in vascular tissue regeneration is effective wound healing that can be resolved by an innovative targeted nanoshuttle that delivers growth factors to blood vessels. This study investigates the production and efficacy of transforming growth factor-β1 (TGFβ1) gene delivery using poly(lactic--glycolic acid) (PLGA) baculovirus (BV) nanoshuttles (NSs). They exhibited an encapsulation efficiency of 86.23% ± 0.65% and a negative zeta potential of -29.57 ± 1.27 mV. In vitro studies in human umbilical vein endothelial cells (HUVECs) revealed that a 12 h incubation period optimized virus transduction. The safety and superior intracellular uptake of NSs and BVs in HUVECs were observed. The NSs carrying 100 and 400 MOI exhibited the highest cell proliferation rates in HUVECs. These sustained-release NSs significantly improved vascular cell migration and wound closure compared to free TGFβ1 carrying BV and can be a groundbreaking find in regenerative medicine, cardiovascular diseases, and chronic ulcer conditions.

摘要

血管组织再生的主要挑战之一是有效的伤口愈合，而一种能够将生长因子输送到血管的创新靶向纳米载体可以解决这一问题。本研究调查了使用聚乳酸-乙醇酸共聚物（PLGA）杆状病毒（BV）纳米载体（NSs）进行转化生长因子-β1（TGFβ1）基因递送的生产和效果。它们的包封率为86.23%±0.65%，ζ电位为-29.57±1.27 mV。在人脐静脉内皮细胞（HUVECs）中的体外研究表明，12小时的孵育期可优化病毒转导。观察到NSs和BVs在HUVECs中的安全性和优异的细胞内摄取。携带100和400感染复数（MOI）的NSs在HUVECs中表现出最高的细胞增殖率。与携带游离TGFβ1的BV相比，这些缓释NSs显著改善了血管细胞迁移和伤口闭合，可能是再生医学、心血管疾病和慢性溃疡疾病领域的一项开创性发现。

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Pharmaceuticals (Basel). 2022-11-10

[6]
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Expert Rev Clin Pharmacol. 2022-11

[7]
Use of a Baculovirus-Mammalian Cell Expression-System for Expression of Drug-Metabolizing Enzymes: Optimization of Infection With a Focus on Cytochrome P450 3A4.

Front Pharmacol. 2022-2-22

[8]
Fluorescently Labeled PLGA Nanoparticles for Visualization In Vitro and In Vivo: The Importance of Dye Properties.

Pharmaceutics. 2021-7-27

[9]
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[10]
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