Schaly Sabrina, Ghebretatios Merry, Prakash Satya
Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, Montreal, Quebec, H3A 2B4, Canada.
Biologics. 2021 Apr 28;15:115-132. doi: 10.2147/BTT.S292692. eCollection 2021.
This review will outline the role of baculoviruses in gene therapy and future potential in personalized medicine. Baculoviruses are a safe, non-toxic, non-integrative vector with a large cloning capacity. Baculoviruses are also a highly adaptable, low-cost vector with a broad tissue and host tropism due to their ability to infect both quiescent and proliferating cells. Moreover, they only replicate in insect cells, not mammalian cells, improving their biosafety. The beneficial properties of baculoviruses make it an attractive option for gene delivery. The use of baculoviruses in gene therapy has advanced significantly, contributing to vaccine production, anti-cancer therapies and regenerative medicine. Currently, baculoviruses are primarily used for recombinant protein production and vaccines. This review will also discuss methods to optimize baculoviruses protein production and mammalian cell entry, limitations and potential for gene therapy and personalized medicine. Limitations such as transient gene expression, complement activation and virus fragility are discussed in details as they can be overcome through further genetic modifications and other methods. This review concludes that baculoviruses are an excllent candidate for gene therapy, personalized medicine and other biotherapeutic applications.
本综述将概述杆状病毒在基因治疗中的作用以及在个性化医疗中的未来潜力。杆状病毒是一种安全、无毒、非整合性载体，具有较大的克隆能力。杆状病毒也是一种高度适应性强、低成本的载体，由于其能够感染静止和增殖细胞，具有广泛的组织和宿主嗜性。此外，它们仅在昆虫细胞中复制，不在哺乳动物细胞中复制，提高了其生物安全性。杆状病毒的有益特性使其成为基因递送的有吸引力选择。杆状病毒在基因治疗中的应用已取得显著进展，有助于疫苗生产、抗癌治疗和再生医学。目前，杆状病毒主要用于重组蛋白生产和疫苗。本综述还将讨论优化杆状病毒蛋白生产和进入哺乳动物细胞的方法、基因治疗和个性化医疗的局限性及潜力。诸如瞬时基因表达、补体激活和病毒脆弱性等局限性将详细讨论，因为它们可通过进一步的基因改造和其他方法克服。本综述得出结论，杆状病毒是基因治疗、个性化医疗和其他生物治疗应用的优秀候选者。
