Department of Plague and Brucellosis, Shaanxi Center for Disease Control and Prevention, Xi'an, China.
National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Front Cell Infect Microbiol. 2024 Oct 30;14:1452143. doi: 10.3389/fcimb.2024.1452143. eCollection 2024.
Human brucellosis is a severe public concern in Shaanxi Province, China, and investigating the epidemiological relationship and transmission pattern of is necessary to devise control strategies.
In this study, a conventional bio-typing approach and whole genome sequencing of single-nucleotide polymorphisms (SNPs) were employed to identify 189 strains.
Based on the conventional bio-typing, 189 strains were identified as , of which 14 were in bv. 1, 145 were in bv. 3, and 30 were variant, and the strains were distributed in all ten cities in Shaanxi Province. SNP analysis was used to identify genetic variation in 189 genomes, and maximum-likelihood was used to generate a phylogeny that identified two clades (A and B) and 19 sequence types (STs). The two clades were highly diverse and exclusively of Eastern Mediterranean origin. Clade B contained 18 STs (2-19), with most isolates originating from a broad swath, implying that multiple lineages circulated in Shaanxi. The 19 STs were composed of 3 to 46 strains isolated from different counties and years, suggesting that multiple cross-county brucellosis outbreak events are driven by multiple lineages. Global phylogenetic analysis revealed that clade A was close to GTIIb, and clade B was placed in the GTIIh lineage, expanding the known diversity of from China.
The human brucellosis epidemic in Shaanxi is driven by multiple indigenous circulating lineages, the knowledge of which will contribute to devising a control strategy and providing the foundation for a comprehensive regional phylogeny of this important zoonotic pathogen.
在中国陕西省，人类布鲁氏菌病是一个严重的公共卫生关注点，有必要调查 的流行病学关系和传播模式，以制定控制策略。
本研究采用传统的生物分型方法和单核苷酸多态性（SNP）全基因组测序来鉴定 189 株 。
根据传统的生物分型，鉴定出 189 株 ，其中 14 株属于 bv.1，145 株属于 bv.3，30 株属于变异株，这些 菌株分布在陕西省的十个城市。SNP 分析用于鉴定 189 个基因组的遗传变异，最大似然法用于生成系统发育树，该树确定了两个进化枝（A 和 B）和 19 个序列型（ST）。这两个进化枝具有高度多样性，仅源自东地中海地区。B 进化枝包含 18 个 ST（2-19），大多数分离株源自广泛的地域，表明在陕西存在多种 谱系循环。19 个 ST 由来自不同县和年份的 3 至 46 株分离株组成，表明多种跨县布鲁氏菌病暴发事件是由多种 谱系驱动的。全球系统发育分析显示，A 进化枝与 GTIIb 接近，B 进化枝位于 GTIIh 谱系中，扩展了中国已知的 多样性。
陕西的人类布鲁氏菌病流行是由多种本土循环的 谱系驱动的，这些知识将有助于制定控制策略，并为该重要人畜共患病病原体的全面区域系统发育提供基础。
