Key Laboratory of Animal Biosafe Risk Prevention and Control (North), Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
College of Veterinary Medicine, Xinjiang Agricultural University, Urumuqi, 830052, China.
Ann Clin Microbiol Antimicrob. 2024 Aug 10;23(1):71. doi: 10.1186/s12941-024-00724-0.
Brucella spp. are facultative intracellular pathogens that cause zoonosis- brucellosis worldwide. There has been a trend of the re-emergence of brucellosis worldwide in recent years. The epidemic situation of brucellosis is serious in Xinjiang. To analyze the epidemic situation of Brucella spp. in Xinjiang among humans and animals, this study identified 144 Brucella isolates from Xinjiang using classical identification and 16 S rRNA sequencing. MLVA, drug resistance testing, and wgSNP detection were also performed. At the same time, analysis was conducted based on the published data of Brucella isolates worldwide. The results showed that the dominant species was B. melitensis biovar 3, which belonged to GT42 (MLVA-8 typing) and the East Mediterranean lineage. The correlation among isolates was high both in humans or animals. The isolates in Xinjiang exhibited higher polymorphism compared to other locations in China, with polymorphism increasing each year since 2010. No amikacin/kanamycin-resistant strains were detected, but six rifampicin-intermediate isolates were identified without rpoB gene variation. The NJ tree of the wgSNP results indicated that there were three main complexes of the B. melitensis epidemic in Xinjiang. Based on the results of this study, the prevention and control of brucellosis in Xinjiang should focus on B. melitensis, particularly strains belonging to B. melitensis bv.3 GT42 (MLVA-8 typing) and East Mediterranean lineage. Additionally, the rifampicin- and trimethoprim-sulfamethoxazole- resistance of isolates in Xinjiang should be closely monitored to avoid compromising the therapeutic efficacy and causing greater losses. These results provide essential data for the prevention and control of brucellosis in Xinjiang and China. Although the isolates from Xinjiang have significant characteristics among Chinese isolates and can reflect the epidemiological situation of brucellosis in China to some extent, this study cannot represent the characteristics of isolates from other regions.
布鲁氏菌属是兼性细胞内病原体，可引起全球范围内的人畜共患病——布鲁氏菌病。近年来，全球布鲁氏菌病呈重新出现的趋势。新疆的布鲁氏菌病疫情严重。为分析新疆人间和动物布鲁氏菌属的流行情况，本研究采用经典鉴定和 16S rRNA 测序从新疆鉴定了 144 株布鲁氏菌。还进行了 MLVA、耐药性检测和 wgSNP 检测。同时，基于全球布鲁氏菌分离株的已发表数据进行了分析。结果表明，优势种为 B. melitensis biovar 3，属于 GT42（MLVA-8 分型）和东地中海谱系。人和动物之间的分离株相关性很高。新疆分离株的多态性高于中国其他地区，自 2010 年以来每年都在增加。未检测到阿米卡星/卡那霉素耐药株，但鉴定出 6 株利福平中介株，rpoB 基因无变异。wgSNP 结果的 NJ 树表明，新疆有三个主要的 B. melitensis 流行复合体。根据本研究结果，新疆布鲁氏菌病的防控应重点关注 B. melitensis，特别是属于 B. melitensis bv.3 GT42（MLVA-8 分型）和东地中海谱系的菌株。此外，应密切监测新疆分离株的利福平耐药性和复方磺胺甲噁唑耐药性，以避免影响治疗效果并造成更大损失。这些结果为新疆和中国布鲁氏菌病的防控提供了重要数据。尽管新疆分离株在中国分离株中有显著特征，在一定程度上可以反映中国布鲁氏菌病的流行情况，但本研究不能代表其他地区分离株的特征。
