Clinic for Oral and Maxillofacial Surgery, University Hospital Schleswig-Holstein, Campus Kiel, Kiel, Germany.
Chair for Multicomponent Materials, Technical Faculty, Christian-Albrechts-University, Kiel, Germany.
Int J Implant Dent. 2024 Nov 14;10(1):53. doi: 10.1186/s40729-024-00576-5.
Wound healing disorders caused by bacterial infections in dental surgery, especially where membranes are used, are a common issue in oral surgery. Cold atmospheric plasma (CAP) offers a non-invasive solution for surface decontamination, including dental implants. The aim of this study was to evaluate the antibacterial effectiveness of CAP on various clinically applied membranes made of collagen and polytetrafluoroethylene (PTFE).
To assess the antibacterial properties of CAP, enterococcus faecalis were seeded on different membranes: Memlock (collagen), Memlock Pliable (collagen), Agronaut (collagen), and PermaPro (PTFE); n = 4. After in vitro cultivation for 6 days, CAP using a kINPen MED with an output of 5 W was applied 5 min and 10 min. Bacterial colony-forming units (CFU) were quantified to detect decontamination effectiveness. In addition, live and dead staining as well as scanning electron microscopy (SEM) of membranes was performed for validation and surface texture analysis.
Bacterial colonization was highest on collagen-based membranes (CFU Memlock: 14.38 ± 8.91). The results showed that CAP significantly reduced bacterial colonization on all membrane types after 10 min application of CAP; Memlock (CFU after 10 min 0.22 ± 0.16^10; p = 0.0256), Argonaut (CFU after 10 min 0.02 ± 0.01^10; p = 0.0129) and PermaPro (complete bacterial decontamination; p = 0.0058). This was paralleled by fluorescence and scanning electron microscopy. CAP was most effective on smooth membrane surfaces as SEM revealed.
CAP thus offers a non-invasive, cost-effective method to reduce bacterial infections in guided bone regeneration using membranes.
口腔外科中，尤其是使用膜的情况下，由细菌感染引起的伤口愈合障碍是一个常见问题。冷等离子体（CAP）为表面去污提供了一种非侵入性的解决方案，包括牙科植入物。本研究旨在评估 CAP 对各种临床应用的胶原蛋白和聚四氟乙烯（PTFE）膜的抗菌效果。
为了评估 CAP 的抗菌性能，将粪肠球菌接种在不同的膜上：Memlock（胶原蛋白）、Memlock Pliable（胶原蛋白）、Agronaut（胶原蛋白）和 PermaPro（PTFE）；n=4。体外培养 6 天后，使用输出功率为 5 W 的 kINPen MED 进行 CAP 处理，时间为 5 分钟和 10 分钟。通过定量细菌集落形成单位（CFU）来检测去污效果。此外，还对膜进行了活死染色和扫描电子显微镜（SEM）检查，以进行验证和表面纹理分析。
基于胶原蛋白的膜上细菌定植最多（CFU Memlock：14.38±8.91）。结果表明，CAP 处理 10 分钟后，所有膜类型的细菌定植均显著减少；Memlock（CFU 处理 10 分钟后 0.22±0.16^10；p=0.0256）、Argonaut（CFU 处理 10 分钟后 0.02±0.01^10；p=0.0129）和 PermaPro（完全细菌去污；p=0.0058）。荧光和扫描电子显微镜也证实了这一点。CAP 在光滑的膜表面上效果最佳，SEM 显示。
因此，CAP 为使用膜进行引导骨再生时减少细菌感染提供了一种非侵入性、具有成本效益的方法。
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