费菜提取物通过清除 ROS 和抑制果蝇体内多条信号通路改善肠道损伤。

Extracts ofHylotelephiumerythrostictum (miq.) H. Ohba ameliorate intestinal injury by scavenging ROS and inhibiting multiple signaling pathways in Drosophila.

机构信息

College of Life Science, Northeast Forestry University, Harbin, Heilongjiang Province, China.

College of LifeScience, Kim Il Sung University, Pyongyang, Democratic People's Republic of Korea.

出版信息

BMC Complement Med Ther. 2024 Nov 14;24(1):397. doi: 10.1186/s12906-024-04686-w.

DOI:10.1186/s12906-024-04686-w

PMID:39543569

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11566468/

Abstract

BACKGROUND

The intestinal epithelial barrier is the first line of defense against pathogens and noxious substances entering the body from the outside world. Through proliferation and differentiation, intestinal stem cells play vital roles in tissue regeneration, repair, and the maintenance of intestinal homeostasis. Inflammatory bowel disease (IBD) is caused by the disruption of intestinal homeostasis through the invasion of toxic compounds and pathogenic microorganisms. Hylotelephium erythrostictum (Miq.) H. Ohba (H. erythrostictum) is a plant with diverse pharmacological properties, including antioxidant, anti-inflammatory, antidiabetic, and antirheumatic properties. However, the roles of H. erythrostictum and its bioactive compounds in the treatment of intestinal injury are unknown.

METHODS

We examined the protective effects of H. erythrostictum water extract (HEWE) and H. erythrostictum butanol extract (HEBE) on Drosophila intestinal injury caused by dextran sodium sulfate (DSS) or Erwinia carotovoracarotovora 15 (Ecc15).

RESULTS

Our findings demonstrated that both HEWE and HEBE significantly prolonged the lifespan of flies fed toxic compounds, reduced cell mortality, and maintained intestinal integrity and gut acid‒base homeostasis. Furthermore, both HEWE and HEBE eliminated DSS-induced ROS accumulation, alleviated the increases in antimicrobial peptides(AMPs) and intestinal lipid droplets caused by Ecc15 infection, and prevented excessive ISC proliferation and differentiation by inhibiting the JNK, EGFR, and JAK/STAT pathways. In addition, they reversed the significant changes in the proportions of the gut microbiota induced by DSS. The bioactive compounds contained in H. erythrostictum extracts have sufficient potential for use as natural therapeutic agents for the treatment of IBD in humans.

CONCLUSION

Our results suggest that HEWE and HEBE are highly effective in reducing intestinal inflammation and thus have the potential to be viable therapeutic agents for the treatment of gut inflammation.

CLINICAL TRIAL NUMBER

Not applicable.

摘要

背景

肠道上皮屏障是防止病原体和有害物质从外界进入体内的第一道防线。通过增殖和分化，肠道干细胞在组织再生、修复和维持肠道内稳态方面发挥着重要作用。炎症性肠病（IBD）是由有毒化合物和致病微生物的入侵破坏肠道内稳态引起的。红景天（Hylotelephium erythrostictum（Miq.）H. Ohba）是一种具有多种药理作用的植物，包括抗氧化、抗炎、抗糖尿病和抗风湿作用。然而，红景天及其生物活性化合物在治疗肠道损伤中的作用尚不清楚。

方法

我们研究了红景天水提取物（HEWE）和红景天正丁醇提取物（HEBE）对葡聚糖硫酸钠（DSS）或胡萝卜软腐果胶杆菌 15（Ecc15）诱导的果蝇肠道损伤的保护作用。

结果

我们的研究结果表明，HEWE 和 HEBE 均显著延长了喂食有毒化合物的果蝇的寿命，降低了细胞死亡率，并维持了肠道完整性和肠道酸碱平衡。此外，HEWE 和 HEBE 消除了 DSS 诱导的 ROS 积累，减轻了 Ecc15 感染引起的抗菌肽（AMPs）和肠道脂质滴的增加，并通过抑制 JNK、EGFR 和 JAK/STAT 通路防止了 ISC 的过度增殖和分化。此外，它们还逆转了 DSS 引起的肠道微生物群比例的显著变化。红景天提取物中含有的生物活性化合物具有作为治疗人类 IBD 的天然治疗剂的充分潜力。